Monday Morning


W-01 CMAR Prep Course
(8-hour workshop continued Tuesday 8:00 AM)
8:00 AM - 12:00 PM/Room: 8C
Leader: Diana P Baumann
Faculty: Diana P Baumann, Camellia M Symonowicz
Facilitator: Sarah J Gilliam
Workshop Fee: $140 Workshop Limit: 50

We are heroes to millions of people and animals, and our work makes a profound difference in this world. As leaders, we have a responsibility to support and drive our employees and operations effectively, efficiently, and compassionately. CMAR certification provides us with a unique set of knowledge and tools. The Laboratory Animal Management Association (LAMA) has developed a workshop for the CMAR exams, designed for your success. Please join us for our preparatory workshop for the Animal Resource (AR) exam. Topics covered include effective management styles and motivating the workforce, training and education for laboratory animal professionals, managing physical resources, policy development, managing budgets, veterinary care, IACUC, and managing compliance. This prep course alone will not prepare you for the AR exam, but provides a refresher and review of your existing studies.

W-02 Developing a Cost Accounting System for Your Animal Facility
8:00 AM - 12:00 PM/Room: 7
Leader/Faculty: David G Baker
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 50

Participants will learn the basic concepts and practical applications of developing a flexible, robust cost accounting and analysis system for their own research animal resource facility. The system developed will be Excel-based and will prove useful for rate (per diem) setting, as well as for other decision-making and forecasting functions of their facility. Participants will be instructed in advance to bring certain facility and personnel data with them in order to maximize the use of the time and allow them to begin developing their own system in the workshop. Participants will include animal facility directors, financial managers, and other vivarium leadership personnel.

W-03A Microsurgery Skills Training Using Surgical Loupes
(offered twice, also Monday 1:00 PM)
8:00 AM - 12:00 PM/Room: 10B
Leader: Robert F Hoyt Jr
Faculty: Tannia S Clark, Tanya L Herzog, Tim J Hunt, Kenneth R Jeffries, Karen Keeran, Audrey Noguchi, Gayle Nugent, Tom Thomas, Art D Zetts
Facilitator: TBN
Workshop Fee: $250 Workshop Limit: 20

Performing surgical procedures with the aid of magnification has gained widespread use in human medicine over the past 30 years. Using surgical loupes, surgeons can now routinely perform procedures on small structures that were considered impossible only a few years ago. Within the past 20 years, the use of magnification has also spread to other health care disciplines, including dentists, dermatologists, and nurses, to increase precision, such that it is considered the standard of care. The use of microsurgery and its importance to biomedical research has only just begun to be realized. Because of their small body structures, rats and mice have generally not been considered as models for many types of surgical procedures routinely performed in biomedical research. Investigators elected to uselarger species (such as dogs, pigs, or nonhuman primates) for such modeling because surgical support equipment is more readily available and the techniques are more familiar. The recent shift in using genetically engineered rodents, especially mice, has now resulted in increased researcher desires to use these animals in more sophisticated modeling procedures, especially surgery. Rather than being limited to only simple procedures (e.g., IM, IP, or IV injections) researchers using microsurgery can now perform complex procedures on many rodent organ systems, such as the heart, lungs, and the gastrointestinal tract. This workshop will provide an introduction to the basic techniques, equipment, and general applications of microsurgery using surgical loupes. Hands-on training will be conducted in 2 phases: teaching students to develop technical skills with exercises using surgical loupes and applying these skills to perform simple surgical procedures using rodent surrogates. We have an enhanced teacher:student ratio for this workshop to increase success.

This Workshop is sponsored in part by Midwest Veterinary Supply; Surgitel Systems, a Division of General Scientific Corporation; SurgiReal and RICA Surgical Products, Inc.

W-04 Play-Doh Pinkies, Squooshey Mice, and Jeopardy: Techniques for Training Husbandry Technicians in a Classroom Setting
8:00 AM - 12:00 PM/Room: 4B
Leaders: Kaile J Bennett, Jayson L Egeler, LaJuanda M Carter
Faculty: Kaile J Bennett, Jayson L Egeler, LaJuanda M Carter, Melissa C Dyson
Facilitator: Jennifer M Schmidt
Workshop Fee: $140 Workshop Limit: 25

Training a large number of husbandry employees simultaneously can be a difficult proposition. Due to increased staffing requirements at our facility, we adapted a training program normally used for 1-2 trainees to a group consisting of 8-10 new rodent husbandry technicians. Accommodating this amount of trainees at one time required us to rethink our previous husbandry training structure. Limitations on the number of trainers and animal rooms available for training led us to develop a unique curriculum which incorporated classroom style learning and activities that complemented traditional in-room training. We observed a successful transition of skills learned in the classroom to application in the animal rooms. Other benefits included increased comradery between trainees, higher number of individuals completing training, and increased involvement from current husbandry staff as mentors. In this workshop, we share our successes as well as hurdles encountered with developing and implementing this program. Participants will have the opportunity to participate in hands-on activities we use in our program. Attendees will receive materials that outline our curriculum, as well as activity and lesson plans. There will also be time dedicated to discuss how to apply aspects of this program to the participant’s home institutions and address specific questions. The target audience for this workshop should be trainers, managers, supervisors, and anyone else involved in the training process who would like to see a new twist on husbandry training.

W-05 Technician to Supervisor 101: The Ups and Downs of Managing People
8:00 AM - 12:00 PM/Room: 5A
Leader/Faculty: Stephen T Baker
Facilitator: Leah M Curtin
Workshop Fee: $140 Workshop Limit: 50

When you excel at a technical skill or provide superior husbandry/study support, individuals are often rewarded with a promotion that expands their role and responsibility to include managing others. Individuals whose primary focus/expertise are animals are asked to change gears and channel their soft skills. This workshop will provide a high-level overview of key concepts to support a successful transition. Topics will include performance management, effective interviewing skills, hiring and firing, how to handle conflict, coaching versus delegating, and how to communicate effectively. This interactive workshop will take participants through various exercises, role plays, and what-if scenarios to provide take-aways which can be applied back at their place of employment. The targeted audience includes new supervisors or first-level managers. If you are new to the world of managing others or contemplating getting into management, you do not want to miss this workshop.

This Workshop is sponsored in part by Pfizer, Inc.


A Review of International Rodent Sentinel Programs with a New Technical Focus
8:00 AM - 10:15 AM/Room: 9B
Leader/Moderator: John J Hasenau
Facilitator: TBN

Rodent sentinel monitoring programs have been evolving for many years. One of the most recent advancements has been exhaust air dust (EAD) monitoring. Te evolution has been driven by technical abilities to evaluate at the rack levels and at the room levels using different ways of sampling. This seminar will be a review from international colleagues on how EAD sampling is being applied at their institutions/organizations. The overall benefits and limitations will be presented and discussed. The incorporation of EAD testing in these international sentinel programs has greatly promoted achievement of the 3Rs. Initial discussion will review the history and explain some types of rodent sentinel systems that are in use and why they are necessary. Then we will evaluate the needs, practicality, the realized benefits, and the outcomes of EAD additions into sentinel programs in the research community. Both mouse and rat sentinel testing will be discussed. A multicenter approach was used for the Italian, German, and English facilities for testing at the rack level of sampling, and a centralized level of sampling was done in Canada. Individuals from different institutions will present their programs and how the incorporation of the EAD into their programs has altered their programs and their use of live sentinels. This seminar is for technicians, managers, veterinarians, and administration who wish to better understand sentinel monitoring and international sentinel programs. The 3Rs will be emphasized throughout the seminar.


8:00 John J HasenauWelcome and Introductions
8:05 John J HasenauHistorical Review of Rodent Sentinel Monitoring Programs
8:25 Alberto Gobbi How the Health Monitoring Program of Our Mouse Facility Is Improved by EAD sampling
8:55 Markus BrielmeierDo Soiled Bedding Sentinels Provide Any Beneficial Information Compared to EAD Samples?”
9:25 Graham MorrisseyIncorporation of EAD into a Rat Sentinel Program
9:45 Lise R PhaneufComparison of Exhaust Air Dust Testing of a Centralized IVC System to a Traditional Sentinel-Based Rodent Health Monitoring Program
10:05 All  Discussion

Good Laboratory Practice in the Academic Environment: What it Is, How to Implement, and How to Compare to a Contract Research Organization
8:00 AM - 10:15 AM/Room: Ballroom D
Leader: Joanne L Zahorsky-Reeves
Moderator: Trinka W Adamson
Facilitator: Roxanne M Morales

Good Laboratory Practice (GLP) is a regulated system concerned with the organizational process and the conditions under which nonclinical studies are planned, performed, monitored, recorded, archived, and reported under the Food and Drug Administration Code of Federal Regulations 21 Part 58. In this seminar, our first speaker will provide a brief history of GLP (why these regulations were promulgated) and detail the components that are necessary for its successful undertaking. Our second speaker will give her experience of creating a GLP-compliant testing facility at a major university, including the trials that have entailed, and comment on what degree of success this university has had. Our third speaker has a background in GLP at both a university and at a contract research organization (CRO), and will give perspective from each. Our concluding speaker, who has been a study director for GLP studies in both environments, will present his insight on what sorts of studies may be best done at each type of facility. Our target audience consists of veterinarians, veterinary technicians, operations/facility managers, and researchers. Audience members will learn what GLP is, what groundwork is required to get this sort of regulated program established in an academic setting, and leave with a better understanding of whether or not they are willing to invest the time, effort, and resources required on behalf of their researchers.


8:00 Joanne Zahorsky-Reeves Welcome and Introduction
8:15 Philippe Baneux GLPs and Quality Assurance Systems: The Basics for Academic Settings
8:35 Joanne Zahorsky-Reeves Establishing and Running GLP in an Academic Environment: How We’re Doing So Far
9:00 Heather Sidener Should We Start GLP at Our University? Perspective from a CRO Veteran
9:25 James G McCabe The Pros and Cons of Initiating a GLP Study in a University Setting Versus a CRO
9:45 All Discussion

Pairing Strategies for Adult Macaques
8:00 AM - 10:15 AM/Room: 18B
Leader: Keely McGrew
Moderator: Michele Wilkinson
Facilitator: Nicole Monts De Oca

This seminar will focus on methods used across different types of facilities to pair-house adult male macaques (Macaca fascicularis and mulatta). Participants will learn methodology for selection of prospective partners, introduction techniques, and strategies to build and maintain relationships. Behavioral indicators of compatibility will be discussed. The differing techniques covered in this seminar include partner selection by temperament, by instinct, by size disparity, or study criteria. In addition to same-sex pairing, the seminar will also discuss alternate paradigms to isosexual pairs: social housing adult males with ovarioectomized females and vasectomized males with intact females, in both pairs and trios. The audience that would benefit from this seminar includes technicians, veterinarians, and behavior managers from NHP facilities who would like to improve or start a social housing program for NHPs.


8:00 Keely McGrew Welcome and Introduction
8:10 Keely McGrew Partner Selection and Introduction Methods
8:30 Melissa M Dragon Social Housing Mature Males: Clues and Cues
8:55 Jennifer N Camacho A Step-By-Step Approach to Pair Housing and Building Relationships with Macaques
9:20 Carolyn M Allen A Cyno Love Connection: Successful Pairing of Aged Male Cynomolgus Macaques with Ovariectomized Females
9:45 Kristen A Flora Mixed-Sex Socializations and Staff Training to Support Relationships

Recent Advances in Genome Engineering in Rodent and Nonrodent Species

8:00 AM - 10:15 AM/Room: Ballroom F
Leader: Monika A Burns
Moderator: Hilda R Holcombe
Facilitator: Erin Bryant

This seminar is designed for researchers working with genetically modified animals and veterinarians responsible for their care. First, CRISPR technology will be reviewed, including a comparison with traditional methods for modification of mouse genomes. The audience will learn about the advantages and potential drawbacks of using CRISPRs to produce mouse models. A brief review of case studies will provide examples of the various approaches CRISPRs can be used to generate genetically modified animals. Additional presentations will focus primarily on primate models. The audience will learn protocols for establishment and support of transgenic nonhuman primate colonies, including required equipment, procedures, and common clinical issues encountered during the process of generating transgenic marmosets. The focus of this presentation will give background and context for veterinary staff working with transgenic nonhuman primates. Finally, audience members will learn which disease models have been created in nonhuman primate species using various genetic engineering techniques, including CRISPR. Advantages and disadvantages of the use of transgenic NHP models in basic research will be discussed. This presentation will provide a scientist’s perspective on the use of transgenic nonhuman primate models in biomedical research.


8:00 Hilda R Holcombe Welcome and Introduction
8:10 Hilda R Holcombe Introduction to CRISPR Technology
8:20 David Grass Overview of CRISPR Technology Used in Mouse Models
8:50 Monika A Burns Veterinary Support Practices for Transgenic Marmoset Programs
9:15 Qiangge Zhang Genetically Engineered Nonhuman Primate Models of Human Disease
9:35 All Discussion

Special Topic Lectures

Aseptic Technique in Rodent Surgery Made Easy

11:00 AM - 12:00 PM/Room: 9B
Speaker: Marcel I Perret-Gentile
Moderator: Luis M Zorrilla
Facilitator: TBN

Believers in the importance of aseptic technique have dealt with the frustration of trying to implement rodent aseptic surgery in programs. Rodent aseptic surgery application is cumbersome, difficult to illustrate, and difficult to implement. Sources for easy, well-illustrated materials to teach investigators proper technique are rare. This struggle has inspired the creation of easy to follow illustrations to help investigators, veterinarians, trainers, and IACUCs everywhere to implement aseptic rodent surgery skills. This presentation will rely heavily on images and videos where the presenter will walk the audience through very simple and fun steps in the application of aseptic technique. This presentation was previously presented at the 2017 LabRoots Laboratory Animal Sciences Virtual Conference and the Texas Branch of AALAS. Participants wishing to further their skills can register for the “Would You Like to Improve Your Suturing and Rodent Surgery Aseptic Technique?” workshop on Monday, October 16, 2017, 1:00-5:00 p.m. Target audience includes veterinarians, IACUC administrators/committees, trainers, research staff, veterinary technicians, and others.

This Special Topic Lecture is sponsored in part by Kent Scientific Corporation and SurgiReal Products, Inc. and Atramat (a subsidiary of Internactional Farmaceutica, S.A. de C.U.)

Biosafety Fitness: How to Develop and Implement an Effective Biosafety Training Program
11:00 AM - 12:00 PM/Room: 18B
Speaker: Hannah M Payne
Moderator: Kathy Laber
Facilitator: TBN

Human safety should be the first priority in any facility conducting infectious disease research. Establishing a comprehensive training program to include introductory, annual follow-up, and study-specific training is one of the most important ways we ensure safety with biological hazards at the University of Georgia (UGA). Our comprehensive training program, along with the UGA Occupational Health Program, is our foundation for exposure and disease outbreak prevention. UGA’s Animal Health Research Center (AHRC), in collaboration with the Office of Biosafety (OBS), develops and implements this training program to capture the following: BSL-3 biocontainment introductory training; select agent annual training; PAPR/ tight fitting respirator training; facility introductory and annual follow up training; emergency response annual training (evacuations, spills, needle sticks, etc.); access control training; infectious agent training; donning and doffing training; species-specific animal training; standard operating procedure (SOP) training and competency evaluations; and documentation management. This special topic lecture will describe the AHRC’s comprehensive training program, the parameters and methods used to create the program, and how we evaluate and evolve our program. Participants will learn how to develop and evaluate an effective comprehensive biocontainment/biosafety training program using the AHRC program as a model. The target audience includes human resources staff, animal care staff, students, supervisors, and biosafety officers and staff.

Charles C Hunter Lecture: Living among Bats
11:00 AM - 12:00 PM/Room: Ballroom D
Speaker: Dianne Odegard
Moderator: Samm C Bartee
Facilitator: Suzanne V Mounsey

This lecture will cover the many benefits of bats to our environment, as well as the threats that bats face, both old and new. This is a topic that bat conservationists and scientists are working hard to address. Bats are vital to our ecosystems. Seventy percent of bat species worldwide eat insects, providing beneficial insect pest control services. Many species can eat 75% of their body weight in insects every night. Research in recent years has shown that bats save the agriculture industry across the U.S. a minimum of $3.7 billion every year, both in crop damage averted and the need for fewer pesticides. The targeted audience is anyone attending the conference.

This Special Topic Lecture is sponsored in part by Committee for Technician Awareness and Development (CTAD).

The Proven Benefits of Openness: How the UK is Ridding Itself of Animal Rights Extremism and Helping the Public to Understand Animal Research
11:00 AM - 12:00 PM/Room: Ballroom F
Speaker: Wendy Jarrett
Moderator: Paula A Clifford
Facilitator: Kirk Leech

In 2012, public opinion polling in the UK showed that overall acceptance of medical research using animals had dropped by 10 percentage points from 76% to 66%. The biomedical research sector realised that it needed to do something significant in order to help people to understand more about why and how animals are used in research. The result was the Concordat on Openness on Animal Research in the UK, published in May 2014 with 72 signatory organizations, and now with more than 110. Signing the Concordat commits signatory organizations to providing information on their websites and being more open about the animal research they carry out or fund. In the three years that the Concordat has been in place, the UK has seen a step-change in the amount and quality of publicly available information on research using animals. Over the past 10 years, the country has also seen a dramatic reduction in illegal animal rights activity, now at practically negligible levels. The speaker will give a brief overview of how and why the Concordat was developed and will then present several of the many examples of institutional openness and public engagement on animal research that have come about as a result of the Concordat. The current climate surrounding biomedical research in the UK will also be examined – government support, media interest, animal rights activity, and public opinion - and future plans for openness in the UK will be shared. The session will then lead into a Q & A and discussion with the audience on whether any of the UK’s experiences and successes could be translated to the North American context. This lecture will be of interest to animal care staff, facility managers, researchers, and those with a security or communications role.

Monday Afternoon

Panel Discussions

Are We Speaking the Same Language? How the IACUC, Veterinarian, and Investigators Can Communicate

12:30 PM - 2:00 PM/Room: 12B
Leader/Moderator: Ellen Kapsalis
Facilitator: TBN
Panelist: Sari Izenwasser, Elisabeth Koncza, Julia Zaias

The panel will present compliance scenarios that often are viewed and interpreted differently by IACUC personnel, veterinarians, and investigators. Various points of view will be presented and explored. For example, what happens when a PI wants to initiate a procedure that is not IACUC approved and the vet advises the procedure can harm the animals? What happens when a PI treats what he labels a typical side effect (not described in the protocol) of a model, thus alleviating discomfort in the animal? Yet, the vet advises the treatment will cause distress and the IACUC has not approved this treatment. In each scenario, the PI thinks he is doing the right thing and believes the IACUC and the vet are over-regulating. The panel aims to highlight the importance of understanding different perspectives in order to resolve complex issues in the most beneficial manner for all parties. The overarching message is that successful animal programs work best when constructive dialog exists among all stakeholders. The target audience includes IACUC professionals and staff, veterinarians, and principal investigators/research staff.

Don't Get Bent Out of Shape: Designing and Implementing a Robust Ergonomics Program
12:30 PM - 2:00 PM/Room: 19A
Leader/Moderator: Jennifer S Kilpatrick
Facilitator: Paul Chamberlain
Panelist: Elizabeth M Horrigan, Keith A Kun, Nathan A Rogers, Terry Snyder

Job tasks associated with lab animal care can be high risk for both acute and cumulative musculoskeletal injury. Reaching, lifting, prolonged standing, and walking, combined with highly repetitive tasks, compound to increase the risk for work-related discomfort and injury. Injuries of this type have a negative impact on morale leading, to a decrease in the quality and productivity of work and the potential loss of experienced employees. Ergonomic evaluations and interventions can successfully address these problems in a cost-effective manner when implemented properly. Making changes to job organization, tools, training, and behaviors can be challenging when vivarium staff have operated in the same manner for a long period of time. Using a participatory ergonomic approach to identify risks and design proposed changes can be an effective method for successfully overcoming these obstacles. This panel will bring together the committee responsible for assessing, designing, and implementing a participatory ergonomic program for the animal care technicians employed at Massachusetts Institute of Technology’s Division of Comparative Medicine. They will describe what a participatory ergonomic program encompasses, and outline the process for implementing such a program while engaging animal care technicians, supervisors, and managers in the process.

Pathology Quiz Bowl
12:30 PM - 2:00 PM/Room: 17B
Leader/Moderator: Cindy Besch-Williford
Facilitators: Kari L Chesney, Michael K Fink
Panelist: Craig L Franklin, Angela Brice, Cindy Besch-Williford

This panel discussion will consist of an informal review of the pathology of laboratory animals in the form of an image-based quiz. Topics will include lesions of well-described infectious and non-infectious diseases, pathological manifestations of emerging diseases, and selected phenotypic characteristics of important genetically engineered animal models. The images will be educational and challenging to laboratory animal specialists at all levels of pathology expertise. Targeted audience is comparative medicine trainees, laboratory animal veterinarians, pathologists, and scientists. Participants from comparative medicine training programs have the opportunity to receive a fabulous cash prize for the highest score. A participation cash prize is also provided. The comparative medicine trainee with the highest score will be recognized at the Committee for Laboratory Animal Training and Research (CLATR) luncheon on Tuesday. Participants will learn gross and histologic pathology of laboratory animals.

This Panel Discussion is sponsored in part by IDEXX BioResearch and Committee on Laboratory Animal Training and Research (CLATR).

Retirement of Nonhuman Primates in Research
12:30 PM - 2:00 PM/Room: 16A
Leader/Moderator: Rachele McAndrew
Facilitator: Jennapher Lingo VanGilder
Panelist: Jason Robert, Stephen Helms Tillery, Amy Kerwin, Kristina Carter

Retiring laboratory animals has become more popular among the research and lab animal community in recent years. Many institutions have successful adoption programs. However, retiring nonhuman primates can be challenging. Roadblocks include institutional and PI resistance, lack of communication between research labs and sanctuaries, cost, and sanctuary space availability. This panel discussion will focus on the importance of lab animal retirement, discuss some barriers that prevent initiation of a successful retirement program, provide different viewpoints from the research and sanctuary community, and offer practical advice on how to start an NHP retirement program at your institution. The panel will be comprised of an ethicist, a principal investigator, a sanctuary director, and a facility trainer. This discussion will help animal technicians, veterinarians, animal care directors, and administrative staff to start productive discussions at their home institutions.


W-06 Boomers, Gen Xers, and Millennials: Are You Effectively Managing Generational Gaps?

1:00 PM - 5:00 PM/Room: 5A
Leader/Faculty: Jamie Mueller
Facilitator: Trinka W Adamson
Workshop Fee: $140 Workshop Limit: 50

This workshop provides content and discussion to increase your awareness and understanding in working with millennials, as well as managing and reducing generational gaps to improve your work and leadership across generational cultures. Participants will take away information that will increase overall generational awareness and understanding about the issues, such as why millennials work differently than previous generations. Participants will also develop effective strategies for communicating across generations, learn how to adapt style in generationally diverse situations and the skills necessary to do so for organizational and team effectiveness, and learn strategies to resolve conflict and build trust and sustainable relationships across generations. The workshop is targeted to directors and managers who manage team members from different generations and team members who work and collaborate with a variety of different generations.

W-03B Microsurgery Skills Training Using Surgical Loupes
(offered twice, also Monday 8:00 AM)
1:00 PM - 5:00 PM/Room: 10B
Leader: Robert F Hoyt Jr
Faculty: Tannia S Clark, Tanya L Herzog, Tim J Hunt, Kenneth R Jeffries, Karen Keeran, Audrey Noguchi, Gayle Nugent, Tom Thomas, Art D Zetts
Facilitator: TBN
Workshop Fee: $250 Workshop Limit: 20

See Monday AM for description.

This Workshop is sponsored in part by Midwest Veterinary Supply; Surgitel Systems, a Division of General Scientific Corporation; SurgiReal and RICA Surgical Products, Inc.

W-07 Monitoring and Troubleshooting in Large Animal Anesthesia: Tips and Tricks
1:00 PM - 5:00 PM/Room: 4B
Leader/Faculty: Cholawat Pacharinsak
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 30

Anesthesia is often a crucial part of animal research, especially with large animals. To make anesthesia safe and effective, anesthetists have to understand how to monitor it. Such monitoring provides an early warning system, the first step to alert them to take actions before complications become irreversible. Specifically, the goals of anesthetic monitoring are to enhance the animal’s safety by assisting anesthetists in identifying the types of sources of complications. Once they are recognized, anesthetists can initiate appropriate troubleshooting techniques. This dry lab workshop focuses on two goals: understanding commonly used anesthetic monitoring equipment and interpreting the data that equipment provides in pigs, nonhuman primates, rabbits, sheep, dogs, and cats and learning a set of troubleshooting techniques. This workshop is suited for veterinarians, veterinary technicians, IACUC members, and researchers.

This Workshop is sponsored in part by Kent Scientific and VetEquip.

W-08 Step Outside Your Box by Using Your Five Minds
1:00 PM - 5:00 PM/Room: 8B
Leader: Daphne L Molnar
Faculty: Diana P Baumann, Carolyn M Malinowski, Daphne L Molnar, Lisa K Secrest, William L Singleton
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 50

In a time of relentless change, there's only one thing that is certain: new challenges and opportunities will emerge that are virtually unimaginable today. We often require more than just competencies or technical expertise to be successful. How can we know which skills will be required to succeed in the future? Using Howard Gardner's "Five Minds for the Future," this interactive workshop will explore this concept of the disciplinary mind, the synthesizing mind, the creating mind, the respectful mind, and the ethical mind. While it's important to increase your emotional intelligence (EQ), we need to shift from the EQ description toward the five minds prescription in an effort to reduce limitations imposed by being unable to conceptualize beyond a single path. Our hope is to convince each participant to step outside their box, think about a topic in a variety of ways, and develop an innate understanding of how to embrace the ever changing environment. The task of cultivating minds constitutes a major challenge to all individuals who work within such a disciplined industry. This workshop will benefit all attendees.

W-09 Would You Like to Improve Your Suturing and Rodent Surgery Aseptic Technique?
1:00 PM - 5:00 PM/Room: 7
Leaders: Marcel I Perret-Gentil, Szczepan W Baran
Faculty: Szczepan W Baran, Danielle Ewing, Laurie Long, Sarah Newman, Marcel I Perret-Gentil
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 30

You may feel proficient, even confident in performing rodent surgery, however you may be surprised how small improvements can have a huge impact on your animal’s recovery and data. Participants will learn and refine commonly used suture and knot-tying techniques. Additionally, participants will learn how to refine their aseptic technique, provide supportive care, and apply these concepts during surgery. In addition, easy to apply hands-on exercises will be demonstrated that have been shown to significantly improve aseptic technique. The workshop will also focus on appropriate hand-eye coordination to improve suturing skills. A state-of-the art inanimate model will be introduced and used during the suture practice. In this highly interactive workshop, participants will be provided with an overview of basic suturing principles and refinements that will improve and enhance your overall surgical skills. Common errors and complications will be discussed and addressed. This workshop is designed for individuals who have minimal or no skills, but is also a great opportunity for those with considerable experience wanting to upgrade their skills and teach others enhanced technique. To get the most out of this workshop, we strongly recommend participants attend "Rodent Surgery: Aseptic Technique Made Easy," the one-hour special topic lecture prior to this workshop.

This Workshop is sponsored in part by Kent Scientific Corporation and SurgiReal Products, Inc. and Atramat (a subsidiary of Internactional Farmaceutica, S.A. de C.U.)


Lytic Enzymes: Exploring Novel Antimicrobial Therapies and Potential Uses in Laboratory Animal Medicine

2:45 PM - 5:00 PM/Room: Ballroom F
Leader/Moderator: Neil S Lipman
Facilitator: Mariya Gugel

Currently, alternative antimicrobial therapies need to be developed due to the increase in antibiotic resistant bacteria and the development of fewer new antibiotics. Bacteriophages, which are species-specific viruses that infect most known bacteria, are being studied as the source of potential novel antimicrobial agents. At the end of replication and assembly inside a host bacterium, the bacteriophage produce a peptidoglycan hydrolytic enzyme (lysin) that degrades the bacterial cell wall, resulting in hypotonic lysis of the bacterium and release of phage progeny. When applied exogenously, purified forms of these enzymes are likewise able to access the peptidoglycan layer in the Gram-positive cell envelope and produce the same lytic effect. Furthermore, lytic enzymes produced by bacteria have also been studied as potential antimicrobial agents. For example, lysotaphin, a zinc metalloproteinase produced by Staphylococcus simulans can lyse Staphylococcus aureus by disrupting its peptidoglycan layer. In this seminar the speakers will introduce key concepts related to lysin structure and function, discuss their characterization as antimicrobial agents, review animal models used to validate their antimicrobial efficacy, and explore potential uses in laboratory animal medicine. The audience will gain an understanding of how lytic enzymes work, their advantages over antibiotics, and how in vitro and in vivo models are used to validate their efficacy. Potential uses and advantages in treating select bacterial diseases of laboratory animals will also be presented including treatment of Corynebacterium-associated hyperkeratosis (CAH) in mice and methicillin-resistant S. aureus infections in non-human primates. This seminar is targeted to all personnel involved in laboratory animal science including clinicians, animal care staff, and commercial breeders.


2:45 Neil S LipmanWelcome and Introduction
2:55 Vincent A FischettiThe Development of Peptidoglycan Hydrolases as Novel Therapeutics
3:20 Chad EulerKilling Staphylococci in Rat Wound and Surgical Infections Using a Topically Applied Bacteriophage Endolysin
3:40 David M Donovan Phage Lysins to Control Mastitis
4:00 Christopher Cheleuitte-Nieves Lytic Enzyme-Based Therapy for Corynebacterium-Associated Hyperkeratosis (CAH) in Mice and Methicillin-Resistant Staphylococcus aureus (MRSA) Infections of Cranial Implants in Nonhuman Primates

The Laboratory Animal Professional's Guide to the Art and Science Behind Effectively Communicating Animal Research
2:45 PM - 5:00 PM/Room: Ballroom D
Leader: Kelly A Metcalf Pate
Moderator: Logan K France
Facilitator: Meghan S Vermillion

Effective communication is key to the success of all laboratory animal professionals, and to be optimally effective requires the ability to adjust communication style to the target audience. This seminar will use an interactive format to explore the science behind communication, allow us to appreciate the art of effective communication to different populations with whom we commonly interact, and hone all of our communication skills in a number of scenarios typical to animal research. We review general guidelines for effective communication through a series of lectures considering the challenges inherent in and proven strategies for communication between laboratory animal professionals and each other, researchers, and the public. We will consider the different backgrounds and goals with which each population approaches animal research, discuss typical scenarios for which communication are key, and acquaint the audience with the terminology and strategies that facilitate effective communication with each population. Each lecture will be followed with an interactive opportunity for audience members to practice their communication skills in scenarios led by our expert communicator speakers. This seminar series is suitable for all laboratory animal professionals, and will give participants a strong understanding of key strategies for effective communication when interacting with researchers, the public and each other, thus preparing them to confidently talk about their work.


2:45 Kelly A Metcalf Pate Welcome and Introductions
2:50 Cindy A BuckmasterCross-Disciplinary Communications within the LAM Community/Interactive Scenarios to Practice Cross-Disciplinary Communications
3:30 Melanie GrahamCommunicating with Researchers Who Work with Animals/Interactive Scenarios to Practice Communication with Researchers
4:10 Paula A CliffordCommunicating About Animal Research with the Public/Interactive Scenarios to Practice Communication with the Public

Transgenic Pigs in Translational Research
2:45 PM - 5:00 PM/Room: 9B
Leader: Lawrence Schook
Moderator: Kelly D Garcia
Facilitator: Michael Eichner

Translational studies require animal models that closely resemble humans in size, genetic diversity, and developmental. Transgenic pigs have been introduced to address this need and have been used to study organ transplantation, infectious disease, genetic therapy, metabolic disease, and more. This seminar will focus on the development and refinement of large animal models to address research needs in translational studies. Various transgenic pig models that have been developed will be described. Experience with the oncopig will be used to illustrate regulatory and management hurdles faced when working with genetically modified agricultural animals in studies across multiple institutions. Oncopigs are transgenic pigs that carry two Cre-inducible cancer-promoting genes (KRASG12D and P53R176H). These genes are silent, and the animals are phenotypically normal, until induction via injection with a replication incompetent adenoviral vector. After induction, control of cell division is perturbed and tumors form. The learning objectives are to define unmet needs in biomedical translational research and limitations of current models; to describe the genesis, capabilities, and applications of transgenic swine models; to illustrate the practical considerations of implementing a transgenic large animal model program in a multisite academic setting; and to demonstrate the importance of data standards in building a multi-site research infrastructure. Target audience includes veterinarians, veterinary technicians, and industry researchers.


2:45 Larry Schook Welcome and Introduction
2:55 Ron C Gaba Setting the Stage for a Modeling Human Disease: The Need for Large Animal Models
3:20 Eckhard Wolf Sharing Advances on Large Animal Models: Transgenic Pigs and Implications for Future Research
3:50 Larry Schook Past and Future Strategies for Creating Porcine Models
4:20 Kelly D Garcia The Oncopig: A Model to Study Cirrhosis and Hepatocellular Carcinoma

Vivarium Master Planning: Doing More with Less

2:45 PM - 5:00 PM/Room: 18B
Leader: Mike P Mottet
Moderator: Jerry Percifield
Facilitator: TBN

This seminar will include discussions from the leadership at three major Institutions, each of which has embarked on a master planning/consolidation study, to determine and identify opportunities to improve efficiencies in all aspects of their animal programs. At the University of California--San Diego, Dr. Richter has implemented the major consolidation effort that came out of his planning study with the opening of a new, consolidated cage processing center that has enabled the university to consolidate and expand animal housing and procedure spaces by the implementation of a campus wide, central cage processing facility. The University of Michigan is just completing its master plan and will offer in-sites to the challenges associated with conducting a master planning effort within a well-established program. The University of North Carolina completed its master plan more than a year ago, and Dr. Fletcher will discuss how that process was viewed internally and update the attendees on what has transpired since the Master Planning was completed. Mike Mottet, who participated in all of these master planning efforts, will provide an overview of the assessment and master planning processes, the challenges that were confronted, and the benefits seen by the Institutions that have incorporated a master plan into their long term strategies. This seminar will benefit all of those involved in vivarium operations, animal facilities financial strategies and building management, planning of new facilities, and overall institutional planning.


2:45 Jerry Percifield Welcome and Introduction
3:00 Craig A Fletcher How Consolidation Effort Transpires
3:30 Robert C Dysko Consolidation Challenges
4:00 Michael P Mottet Assessment and Consolidation Process
4:30 Philip J Richter Implementation of a Campus Wide Consolidation for a Central Cage Processing Facility