Technical Trade Presentations

Strategic Planning Tips for Finding a Location for Your Vivarium
1:00 PM–1:20 PM /Room: 217C
Speaker: Alicia A Pandimos Maurer
Moderator: Debora Hankinson

Determining the location for a new vivarium is no easy task. Not only are vivarium’s unique buildings, but their physical location can have its own challenges including zoning, permitting, operations, and public relations. CRB’s strategic planning group recently developed a feasibility study for one such organization looking for a new vivarium site. Criteria to guide the process was developed by the planning team, and multiple cities and jurisdictions were evaluated, scored, and ranked. The outcome of this planning study provides great advice and tips for others in similar situations.

This Technical Trade Presentation is sponsored in part by CRB.

Using Technology to Reduce the Cost of Compliance
1:20 PM–1:40 PM /Room: 217C
Speaker: Thane K Plummer
Moderator: Deborah A Benner

Compliance has become a permanent part of the workflow of each organization. As regulations increase, organizations must respond accordingly. Part of the organization’s response is to put processes and procedures into place, often adding software to automate or semi-automate the tasks involved. This software can be both a blessing and a curse. The benefits include data validation and centralization, efficient reporting, and database searching. The downside of a software system comes with many costs far beyond the purchase price. These include deployment costs, training costs, data “lock-in” costs, and time costs, such as decreased efficiency and network/website bottlenecks. In this session, we will show how our Aztech software keeps the benefits and minimizes costs, using a technology called Smart Documents to place important information in a database while allowing researchers or staff members to retain control over their data. Aztech keeps the protocols and important documents in their native format, such as Microsoft Word, and automatically extracts data to the database. This solution can save up to 80% of the time required for compliance, reducing the typical costs of implementation and usage while keeping all the benefits of a database system. As a result of this session, the participant should come away with an understanding of this technology and how it could benefit his or her own organization.

This Technical Trade Presentation is sponsored in part by AppVizo, LLC.

When Is Software Necessary?
1:40 PM – 2:00 PM /Room: 217C
Speaker: Aaron Plante
Moderator: Guy Oliver

In a world of increasing regulatory requirements, facilities that conduct research have the burden to record and report on a large amount of data annually. This burden often becomes greater over time until a tipping point is reached and facilities require assistance. Tracking research for the purpose of regulatory compliance and reporting, as well as day-to-day operations, can become so burdensome the management effort swamps the research effort. In such cases, the question of how to decide whether the implementation of a software solution is an appropriate and effective solution becomes not only relevant, but immediate. This presentation answers the question, “is it time for a software solution?” It also provides the characteristics of a solution that can mitigate the risk of noncompliance and help protect the integrity of the research itself. Participants will learn: indicators that suggest it’s time to consider a software solution; why the indicators suggest software is now necessary or desirable; how software can solve the problems indicators present; and what characteristics to look for in a software solution. The target audience will include, but is not limited to, study/research directors, lab/plant/facility/operations manager, IACUC chair/coordinators, veterinarians, deans, and vice chancellors.

This Technical Trade Presentation is sponsored in part by TOPAZ Technologies LLC.

Eliminate the Bottleneck in Your Life
2:00 PM - 2:20 PM /Room: 217C
Speaker: Ryan Yanase
Moderator: Todd Poley

Rederivation, quarantine breed, wean, biopsy, train, and repeat. With so many animals and so little time, it is time to breathe a sigh of relief. You need automation. Automated genotyping can reduce overcrowding, eliminate redundancy, and minimize headaches that create unnecessary problems for you and labs. Accomplish more by efficiently managing your colonies. Automated genotyping can help produce more scientifically useful animals from the same space. Let us help you help them. Come see how to remove the bottleneck by reducing nonscientifically useful animals from your facility, while improving animal welfare through the elimination of redundant and unnecessary tasks. Automated genotyping can eliminate mouse-keeping as a time constraint and financial burden. Lab animal professionals who participate or oversee breeding services, colony management, and work with labs that require genotyping is the target audience.

This Technical Trade Presentation is sponsored in part by Transnetyx, Inc.

Low-Flow, Digital Vaporizer for Mice and Rats— Safer for You, Safer for Your Animals
2:20 PM–2:40 PM /Room: 217C
Speaker: Ethan Ide
Moderator: David FitzMiller

Many facilities and several traditional vaporizer manufacturers recommend a flow rate between 0.5 and 1L/min to deliver anesthesia to rodents. The recommended flow rate for a 30g mouse is approximately 50 mL/min (2-2.5 x minute volume). A traditional, human-sized vaporizer is not designed to deliver the recommended low flow rates for mice and rats. Using such high flow rates with equipment not designed for use on rodents results in the dangerous exposure of lab personnel to waste anesthetic gas. A low-flow, digital anesthesia system is available that uses >20 times less anesthetic and gas. This system precisely delivers the optimal anesthetic amount for small laboratory animals with minimum WAG. This significantly reduces the possibility of exposure and health concerns to lab personnel and the environment. It is safer for you, your animals, and the earth. You will learn the differences between a high flow and low flow vaporizer, the physiological effects of anesthesia on your animals, and ways to reduce your exposure to waste anesthetic gas. This presentation is ideal for technicians working with anesthetic equipment as well as the investigators doing the study.

This Technical Trade Presentation is sponsored in part by Kent Scientific Corporation.

Exposure Limits for Halogenated Anesthetic Gases: Past, Present, and Future
2:40 PM – 3:00 PM /Room: 217C
Speaker: Brett T Field
Moderator: Milton April

In the past 25 years halogenated anesthetic agents, primarily isoflurane and sevoflurane, have become indispensable tools to laboratory animal science. With the proliferation of rodent specific equipment, virtually every animal laboratory today makes use of these drugs. Since the beginning of their widespread clinical use in the 1960’s, the health effects of halogenated agents have been the subject of numerous studies and by now, the importance of monitoring and limiting operator exposure has become common knowledge. However, what is considered an acceptable level of exposure continues to be the subject of debate and discussion. In the United States, the recommended exposure limits (REL) for isoflurane and sevoflurane are often based upon a decades old report published before the drugs were introduced. Shifting attitudes and new information are leading to updated policies on workplace exposure to the most commonly used agents in this industry. This session will discuss the history of RELs for these drugs, the current state of regulation worldwide, and what to expect for the future. The information in this presentation is relevant to all users of anesthesia equipment as well as lab managers, facility managers, health and safety managers, and veterinarians responsible for supervising the use of anesthesia equipment.

This Technical Trade Presentation is sponsored in part by E-Z Systems / Euthanex Corp.

Microsampling and the 3Rs: The Use of Capillary Micro Sampling (CMS) and EZ-Spot® Dry Whole Blood Spot (DBS) Technologies for Biomarker Analysis and Routine Serosurveillance
3:00 PM–3:20 PM /Room: 217C
Speaker: Rajeev K Dhawan
Moderator: Sarah K Warren

Microsampling techniques including CMS and EZ-Spot® DBS are important tools for routine analysis of biomarkers in discovery studies as well as serosurveillance of rodent colonies. Both use a drop of whole blood and are alternatives to submitting rodent serum for biomarker profiling in discovery studies and/or routine serology screening. Microsampling techniques are important especially in small animals where the amount of blood and/or serum is limited. Biomarkers are important indicators and regulate many processes in progression or state of diseases. Their profiles provide necessary drug efficacy and/or toxicology information in multiple stages of drug development. Microsampling efficacy of hematology and chemistry parameters for various species was tested on whole blood and sera, respectively. These were collected in normal tubes and CMS, and by testing their dilutional linearity by serially diluting them in 10% increments. Five-fold dilution linearity of WBC, RBC, HGB, HCY, PLT hematology parameters was achieved in multiple species including rats and mice. Similar five-fold dilution linearity of most of the clinical chemistry markers was achieved including CHOL, TRIG, HDL, LDL, and FFA in hamster serum. In a mouse study, serum corticosterone levels were found to be comparable to CMS and DBS levels. LOD comparison of eluted antibodies from EZ-Spot® cards versus serum using mouse and rat monospecific antisera showed similar endpoints. Two analysts performed triplicate runs of 16 sera (rats and mice each) and matching EZ-Spot® with data showing no false positives results, i.e. high specificity of both assays. Of the 720 immune sample-assay combinations with expected positive results, 714 (99.2%) of the sera and 702 (97.5%) of EZ-Spot® eluates gave positive net scores confirming comparable analytical and diagnostic sensitivity and specificity, reproducibility and ruggedness. Data from these experiments shows that in accordance with the 3Rs principle overall number of animals used in the studies can be reduced.

This Technical Trade Presentation is sponsored in part by Charles River.

Postsurgical Care and Maintenance of Catheters and Implanted Devices Including Accessing, Flushing, and Locking
3:20 PM–3:40 PM /Room: 217C
Speaker: Candace A Rohde-Johnson
Moderator: Deborah A Benner

One of the most overlooked aspects of catheter success comes after the surgery is complete. Proper technique in accessing the implanted device, as well as appropriate routines for flushing and locking, are essential to the ongoing success of any catheter. In this session, we will focus on the postsurgical care of your catheter including a review of locking solutions, recommended frequency of flushing, aseptic technique for accessing the catheter, and troubleshooting. In addition to the discussion, participants will have the opportunity to observe a video showing proper cleaning, flushing, and locking procedures for the catheter. Throughout the session, participants will be introduced to several types of access devices, including harnesses, buttons, and VAPs and will understand the benefits and limitations of each. This session is ideal for anybody who is new to catheter implantation and maintenance, but can also serve as a great refresher on the best techniques and new products that aid in catheter care and longevity.

This Technical Trade Presentation is sponsored in part by SAI Infusion Technologies.

Helpful Engineering for Happily Handling Huge Rats
3:40 PM – 4:00 PM /Room: 217C
Speaker: John C Schofield
Moderator: Deborah A Benner

The research rat at 800g can be a fearsome beastie for students who lack the courage and confidence to cope. Their formidable dental equipment and quick reflexes demand respect; but help is at hand. This presentation will illustrate some unique and inventive engineering, which gives total confidence and enables risk free restraint of all rat sizes for drug administration, tail blood sampling, or blood pressure measurements. The target audience includes all rat users.

This Technical Trade Presentation is sponsored in part by OTAWOG Systems Limited.

Weighing the Benefits of Zebrafish Diets and Production Performance
4:00 PM – 4:20 PM/Room: 217C
Speaker: Jackie Zimmerman
Moderator: Deborah A Benner

Zebrafish being used as a research model is becoming more popular. Providing an alternative to live feeds for zebrafish culture provides a cost savings as well as a more consistent product to feed your fish. Examine the comparison between costs of live culture of zooplankton, including lab time and resources, to the cost of dry feed. Review the production details and learn more about the benefits of providing a more nutritionally consistent product for zebrafish culture.

This Technical Trade Presentation is sponsored in part by Skretting North America.