Technical Trade Presentations Track 1: Husbandry/Facility Management

Tank Graffiti versus Labels

1:00 PM - 1:20 PM/Room: 16A
Speaker: Robby Davis
Moderator: Deborah A Benner

In Zebrafish research, a common practice is self-created marking of tanks with critical data. But these randomized methods provide a poor communication mechanism. We will explore an alternative approach that enables accurate reproducibility of applied protocols, experiments, or generational breeding/reproduction, yielding statistically validated results by employing concise recorded labeling. Participants will learn an innovative form of accurate communications methods drawn from resident databases producing legible tank labeling that can evolve as subjects age and protocols progress, requiring the ongoing relabeling of tanks as they are relocated around the laboratory. This presentation is relevant to all zebrafish professionals who participate or oversee breeding services, strain management, or work with lab/facility management, as well as grant writers, health and safety managers, IACUC committee members, veterinarians, principle investigators, researchers, and post docs.

This Technical Trade Presentation is sponsored in part by DanioData by Fulcrum.

Keep It Clean with Polymer Flooring
1:20 PM - 1:40 PM/Room: 16A
Speaker: Gordon Yee
Moderator: Deborah A Benner

Your facility is dirty. Your animals are clean, and you want them to stay that way. How? Keep your facility clean, and stop dirt and contamination before it has a chance to get in. It is time to take a cue from other clean industries that rely on polymer flooring technologies to maintain their clean environments. These industries include nanotechnology optics, semiconductor, GLP manufacturing, aerospace, telecom, and many other areas where maintaining a clean environment is paramount to having a reliable product. TechTrak Flooring offers the next generation of particulate capture by providing a completely passive barrier that strips particulate from feet and wheels. It can work in conjunction with or separately from current PPE protocols, and will not only help to keep problematic pathogens and contaminants out of your facility; it will simply help to keep things clean. This is not your father’s sticky mat.

This Technical Trade Presentation is sponsored in part by Ancare.

Animal Watering Myths Exposed
1:40 PM - 2:00 PM/Room: 16A
Speaker: Arnie Markwald
Moderator: Mike R Evans

This will be a lighthearted but poignant look at animal watering myths. How many times have you heard something and accepted it as true? The old axiom of saying something enough times makes it true can create yths. This presentation will take a look at myths surrounding animal drinking water, bottles/bags, flushing systems, recirculating systems, reverse osmosis, green practices, carbon footprints, piping materials, chemical treatment, and many more. Myth examples include “chlorine is bad”, “recirculating systems are green," and “flushing systems waste water." Join us as we explore the world of animal watering and expose common myths surrounding its use so that attendees can make an informed decision when it comes to purchasing an animal watering system.

This Technical Trade Presentation is sponsored in part by Edstrom Industries LLC.

Officialdom and High-Level Disinfection
2:00 PM - 2:20 PM/Room: 16A
Speaker: Kathy Kane
Moderator: Deborah A Benner

The EPA has a very specific definition for disinfection, essentially a six log (99.9999%) reduction of specific challenges in less than 10 minutes. Although many marketing materials claim disinfection, many of those products don’t actually meet those standards. Vivarium operations and biosecurity staff are responsible for disinfection efficacy and need to determine the false claims from the real ones. When it comes to personal exposure limits of chemicals used in disinfection, OSHA has more stringent standards for PAA and H202. The challenge is to determine the best systems for fully high-level, no-touch disinfection of entire treated spaces that meet the efficacy definition of disinfection and at the same time meet or exceed the OSHA PEL safety standards. This presentation is appropriate for lab/plant/facility/vivarium operations managers and biosecurity and safety management.

This Technical Trade Presentation is sponsored in part by Altapure LLC.

Emergent Technologies Help Improve Research Animal Management
2:20 PM - 2:40 PM/Room: 16A
Speaker: Charles Donnelly
Moderator: Julie Morrison

The NIH invests approximately $12 billion each year in animal model research that is central to both understanding basic biological processes and for developing applications directly related to improving human health. Surveys show that over 90% of research organizations still depend heavily on paper notebooks and spreadsheets, or expensive on-premises solutions for capturing and organizing research information. These solutions are often inefficient, error prone, unsecure, limit data sharing, and do not track sufficient information for experimental reproducibility. New approaches to improve laboratory animal welfare, as well as more capable and cost effective husbandry techniques and data management, are high priorities to the NIH. Efficiency gains can translate directly to cost savings, increased scientific output, and reduced burden on research animal subjects. Emergent technologies such as IoT monitors, machine learning algorithms, and mobile devices integrated with global cloud infrastructure, such as Microsoft Azure and Amazon EC2, are revolutionizing how workflows are conducted in nearly every industry. For about the same cost per year as a few mouse cages, cloud hosted systems can offer comprehensive data and workflow management with real-time data capture, data analysis, instant push notification, and multimedia communication backed by nearly limitless compute capacity and geo-redundant data storage. Researchers no longer need to maintain and own expensive and complex IT infrastructure, they simply subscribe to a research data service with 24/7 global availability. Here we discuss scalable cloud solutions for researchers looking to focus on their research, improve their data management, ease their efforts for regulatory compliance, and reduce their IT maintenance costs.

This Technical Trade Presentation is sponsored in part by RockStep Solutions.

Using Technology to Stamp Out Paper Cage Cards, Printers, and Manual Census in the Vivarium
2:40 PM - 3:00 PM/Room: 16A
Speaker: Rich VanDewater
Moderator: Deborah A Benner

The use of paper cage cards and manual census is ubiquitous in every animal facility. But cards can be misplaced and must be reprinted every time information is added or changed. Printers in each housing room generate disruptive noise, take up valuable space, and use expensive toner cartridges. Or long walks are taken to the printer room to retrieve new cards. Cage cards are also used to take cage census but cages must be hand counted or every single card in the facility must be manually scanned. All these processes are time consuming and inefficient. Participants will learn how the CageTalkers® unique technology is used to replace paper cage cards, eliminate printers, archive cage card information, perform automatic cage census, and track and locate individual cages. The target audience is all lab animal professionals who oversee vivarium management and want to learn about innovative streamlining of record keeping and cage census.

This Technical Trade Presentation is sponsored in part by Animal Care Systems, Inc.

Using Data Collection Technology for Cage Census
3:00 PM - 3:20 PM/Room: 16A
Speaker: Daniel Kwoka
Moderator: Neil Amrhein

Animal research facilities throughout the country, whether they are pharmaceutical or university institutions, have an enormous data collection burden on their shoulders. They need absolutely flawless records on the scores of cages that come into and out of the facility. Additionally, cages can be moved throughout a facility on a daily basis. It's like running a hotel for mice. Facilities need a method to track cages easily and accurately. There are several data collection technologies and methods that can be used, but they need to be incorporated properly in order to meet the goals of each unique operation. In this presentation we will compare and contrast the three major technologies being used for cage census (barcode, passive RFID, and active RFID), as well as cover the benefits and tradeoffs of each technology.

This Technical Trade Presentation is sponsored in part by Capturity.

How Cloud Connectivity for Laboratory Equipment Can Revolutionize Lab Productivity
3:20 PM - 3:40 PM/Room: 16A
Speaker: Amit Gupta
Moderator: Deborah A Benner

From adjusting thermostats online to starting a car from an app to viewing security camera video on a website, internet connected devices have already revolutionized the consumer market. In the consumer market, internet connected devices are generically referred to as the Internet of Things (IoT). Similar types of technology and connectivity are already being integrated into laboratory equipment. If this trend continues, within five to 10 years, the laboratory could primarily consist of internet-connected or “smart” equipment. This type of smart equipment enables researchers to be more productive by allowing them to remotely perform experiments, run processes, monitor tests, collect data, and more. Traditionally, cloud connectivity has been a challenge for laboratories because most lab equipment use audible alarms, display messages on a screen, or require input on a terminal. Smart equipment solves the proximity issue and embeds itself into a researcher’s natural workflow by interacting via e-mail, SMS, app, and/or website. Smart lab equipment allows researchers to access and collect critical information as it occurs, thereby, empowering the researcher to focus on the science rather than the equipment. Productivity gains are realized by minimizing downtime, reducing waiting time, and allowing researchers to work on multiple pieces of equipment simultaneous and remotely. This presentation will cover the opportunities presented by a new age of smart laboratory equipment and how labs can leverage smart laboratory equipment to realize increased productivity. Additionally, issues regarding data security and data ownership will be discussed.

This Technical Trade Presentation is sponsored in part by Consolidated Sterilizer Systems.

Unlocking Novel Insights with the Digital Vivarium
3:40 PM - 4:00 PM/Room: 16A
Speaker: David Hutto
Moderator: Deborah A Benner

The utility of animal models in drug development is limited in part by the measures available to researchers for monitoring animals. In life, measures are often subjective, lack clinical relevance, and can be hard to reproduce. Conventional approaches to collecting in-life measures are labor-intensive and may introduce experimental variability, as they require frequent handling of the animals during the course of the study. This session will examine how the ever-increasing combination of existing monitoring technologies with data science, environmental design, and cloud infrastructure is creating new “digital vivariums” of the future that seek to address the long-standing limitations that have existed in in vivo research.

This Technical Trade Presentation is sponsored in part by Vium, Inc.

Technical Trade Presentations Track 2: Novel Techniques/Technologies

The Importance of Establishing a Cryopreservation Program in the Laboratory

1:00 PM - 1:20 PM/Room: 17B
Speaker: Chapell Miller
Moderator: John A Park

Key components of a successful zebrafish facility are trained, committed, and reliable staff; efficiency; predictability; healthy fish; a high level of biosecurity; and a back-up system. All of these factors should be standardized, and a well-working cryopreservation program is a natural component of such standardization. Unfortunately, not all zebrafish facilities have a solid plan for back-up in case of a disaster. Human error, instrument malfunction, power outages, disease outbreaks, and natural disasters are all possible scenarios that demand a solid backup plan. A cryopreservation program is a back-up strategy that will prevent loss of lines in most of these cases. By storing zebrafish lines in the form of cryopreserved sperm samples, preferably at an off-site location, lines can be re-created quickly if needed. In the mean time you can retire lines that are not currently in use and reduce costs and time spent doing husbandry, keeping focus on the research. By increasing available tank space, fish can be stocked at a lower density, thereby reducing health risks. Compared to live lines, cryopreserved sperm samples have a known health and genetic status, with no risk of genetic drift and disease. On average, fish at the age of 12-15 months should be removed from the system to maintain good health. A predictable outcome from the thaw and IVF is important for relying on cryopreservation as a secure back-up. Cryogenetics’ experienced staff can tailor a cryopreservation program for your lab, depending on each facility’s individual needs and set-up. By using the Cryogenetics cryopreservation program the outcome is stable and output will be predictable, thus providing a valuable service option for zebrafish laboratories.

This Technical Trade Presentation is sponsored in part by Cryogenetics, Inc.

Breeding Productivity: Using Technology for the Greater Good
1:20 PM - 1:40 PM/Room: 17B
Speaker: Ryan Yanase
Moderator: John A Park

With rapid advancements in consumer technology that reduce human error, improve communication, and minimize lag time across industries, the question arises of how could the animal research community leverage productivity tools. This discussion will pinpoint two productivity tools and how they could work together to change the way animal research is done: automated genotyping and breeding management software. With these two working together in harmony, institution and their facilities worldwide would see tremendous improvement in the efficiency of working with scientifically useful animals, improvement in communication between facility and researcher, and the reduction of human error entry.

This Technical Trade Presentation is sponsored in part by Transnetyx.

The Humanizable NCG: CRISPR-Generated Triple Immunodeficient Mouse Model
1:40 PM - 2:00 PM/Room: 17B
Speaker: Alice White McVey
Moderator: Gautam Rajpal

The Nod CRISPR Prkdc Il2r gamma mouse, or NCG for short, is a novel triple immunodeficient mouse produced by Charles River. Created by Nanjing University in 2014, the model was in-licensed by Charles River in 2016 and launched to the research community in 2017. With targeted disruptions using CRISPR technology in two critical genes for immune development (Prkdc and Il2rg), the result is a mouse that is T, B, and NK cell deficient. The model is suitable for applications such as oncology, immunology, infectious disease, and diabetes, as well as transplant research. The immunodeficient status of the NCG enables humanization studies including with CD34+ and human peripheral blood mononuclear cells (PBMCs), leading to the development of human immune cell populations. The model can be used to study tumor growth, especially slow growing tumors and patient derived xenografts (PDX). The versatility of the NCG makes it ideal for both basic and clinical research, across academic and commercial institutions.

This Technical Trade Presentation is sponsored in part by Charles River.

Surgery and the 3Rs (Reduction, Refinement, and RFID) How RFID Can Increase the 3Rs while Providing Essential Data to the Researcher
2:00 PM - 2:20 PM/Room: 17B
Speaker: Matthew Ruiter
Moderator: Brad Gien

Surgeons and surgical managers are always looking at ways to maintain the 3Rs. Until now, there has not been a way to efficiently record and process the data from surgery such as times, procedures, success, and the surgeon. Currently, the recording of this surgical data is mostly done by hand, on hard-to-read surgical notes. Now, with the use of RFID microchips and software, the collection of surgical data or, “passive data collection," is as simple as an RFID scan of the surgeon, animal, or item. In a sterile environment, passive collection is critical. In addition to surgical metrics for understanding times and outcomes of surgery, it also benefits the researchers. Having the data collection start at the vendor allows for the simple import of the animal’s ID, as well as other crucial data such as DOB, surgery, sex, drugs provided, and order quantity. The surgical vendor-provided data eliminates duplicate data entry at the end user and increase data integrity of every research project.

This Technical Trade Presentation is sponsored in part by UID Identification Solutions.

A Different Way to Approach Waste Anesthetic Gas
2:20 PM - 2:40 PM/Room: 17B
Speaker: Robert (Bob) J Schrock
Moderator: John A Park

Waste anesthetic gas control is still a frequently discussed topic. You may be surprised to know that there have been no official recommendations to OSHA regarding isoflurane exposure specifically. Some European studies have examined the newer anesthetics (isoflurane, sevoflurane, and desflurane) and have made a case for numbers that are higher than those previously recommended for agents like halothane and methoxyflurane. And so, like the debate about =euthanasia, people will still have a difference of opinion on what is considered appropriate. There are many products that have been designed to mitigate the potential exposure to waste gas, but what if funds are limited? We will discuss issues related to waste gas and explore various means to reduce and eliminate the potential exposure. Does it all involve the purchase of new equipment? The answer may not be what you expected.

This Technical Trade Presentation is sponsored in part by VetEquip, Inc.

Rodent Vital Signs Monitoring System
2:40 PM - 3:00 PM/Room: 17B
Speaker: Anilkumar K Reddy
Moderator: John A Park

Surgical and nonsurgical procedures involving anesthetized rodent subjects require continuous monitoring of heart rate, respiration rate, core temperature, and blood oxygenation to keep the state of the animal as normal and stable as possible. This promotes animal welfare by meeting two of the 3Rs (refinement and reduction), satisfies IACUC monitoring and documentation requirements outlined in The Guide, and minimizes variability in measurements. Continuous monitoring and making adjustments to maintain stable vital signs are especially important during survival surgical procedures. Recent technological advances in monitors permit early detection of potential problems and facilitate key decisions during surgical procedures without creating additional burden on either the animal subject or the surgeon. Decreased mortality, improved recovery, and reduced measurement variability all contribute to better study outcomes. This presentation will discuss how an integrated approach to surgical monitoring can facilitate regulatory compliance and reduce both the workload during surgery and the number of surgeries required. Discussion will include monitoring and control of core body temperature, monitoring of ECG/heart rate, respiration rate and blood oxygenation, and documentation during surgical procedures. The target audience is veterinarians, laboratory animal care professionals, laboratory managers and technicians, and anyone interested in learning how to make surgical monitoring easy and improve subject survival rates.

This Technical Trade Presentation is sponsored in part by Indus Instruments.

Low-Flow, Digital Anesthesia System for Mice and Rats
3:00 PM - 3:20 PM/Room: 17B
Speaker: Ethan Ide
Moderator: David FitzMiller

Many facilities and several traditional vaporizer manufacturers recommend a flow rate between 0.5 and 1L/min to deliver anesthesia to rodents. The recommended flow rate for a 30g mouse is approximately 50 mL/min (2-2.5 x minute volume). A traditional, human-sized vaporizer is not designed to deliver these low flow rates for mice and rats. Running such high flow rates with equipment not designed for use on rodents results in the dangerous exposure of lab personnel to waste anesthetic gas. A low-flow, digital anesthesia system is available that uses a fraction of the anesthetic and gas required by a traditional vaporizer. This system precisely delivers the optimal anesthetic amount for small laboratory animals and creates minimum WAG, significantly reducing the possibility of exposure and health concerns to lab personnel and the environment. You will learn the differences between a high-flow and low-flow vaporizer, the physiological effects of anesthesia on your animals, and ways to reduce your exposue to waste anesthetic gas. This presentation is ideal for technicians working with anesthetic equipment, as well as the investigators doing the study.

This Technical Trade Presentation is sponsored in part by Kent Scientific.

Micro-Fluoroscopy: A New Imaging Modality in Small Animal Research
3:20 PM - 3:40 PM/Room: 17B
Speaker: Gil Zweig
Moderator: John A Park

Small animal-based research projects requiring the use of real-time x-ray presently have available fluoroscopic systems that employ either digital flat panel or Cesium Iodide intensifier x-ray imaging devices. Both devices, it will be seen, exhibit limitations in resolution and image magnification, as well as requiring relatively high radiation dose levels. A fluoroscopic imaging device and system will be discussed, which is shown to exhibit high resolution fluoroscopic images having a high degree of magnification using comparatively low-dose levels of radiation. The essential characteristic of magnification fluoroscopy is that while viewing a 50 millimeter field of view, the video image is a dynamic (moving) real-time fluoroscopic image that can be recorded as a movie and at the same time can be magnified approximately 25 times, much like a fluoroscopic microscope. The x-ray source used has a 10 micron focal spot size to minimize penumbra effect and is continuously energized, operating at an anode voltage of 35 to 40 kilovolts and a current of 150 MicroAmps. The x-ray source is positioned approximately 6 inches from the 50 mm diameter entrance window of the fluoroscope and has a beam limiter that shapes the beam to just fill the 50 mm fluoroscope entrance window. Since the image is intensified, the radiation dose levels required to produce the images are orders of magnitude less than those required by digital flat panel and consequently researchers can be performing surgical procedures on an anesthetized animal with a minimal of protective garments.

A Novel Treatment Option for Primates Suffering From Idiopathic Chronic Diarrhea
3:40 PM - 4:00 PM/Room: 17B
Speaker: Karen Froberg-Fejko
Moderator: Karena Thek

PrimiOtic and PrimiOtic Plus are primate-specific live probiotics containing the primate-derived probiotic bacteria Lactobacillus reuteri, a digestive commensal bacteria in the nonhuman primate. L. reuteri is a natural inhabitant of a healthy gastrointestinal tract that secretes reuterin, which has antimicrobial properties against pathogenic bacteria, such as Salmonella, while keeping the normal gut microflora in tact. L. reuteri also modulates and enhances the immune system. The audience will learn idiopathic chronic diarrhea (ICD) of nonhuman primates is a major gastrointestinal disorder in research animals. ICD is a leading cause of morbidity in rhesus macaques kept in captivity. Probiotics as defined by the World Health Organization are “live microorganisms which when administered in adequate amounts confer a health benefit on the host.” Probiotics have scientifically been demonstrated to support and normalize the gastrointestinal flora to maintain a healthy balanced gut minimizing the incidence of ICD and can be an effective method to decrease the incidence of diarrhea in captive nonhuman primates. Unlike traditional probiotics, the L. reuteri in PrimiOtic and PrimiOtic Plus have been harvested and cultured from the gut of a nonhuman primate. Recent research has identified the importance of host specificity in bacterial probiotics in effectively colonizing the gastrointestinal tract. Specifically, L. reuteri strains isolated from different animal species expressed host-specific genes, some of which were responsible for microbe attachment to the host gut surface. The host specificity of Primiotic and PrimiOtic Plus support the colonization of the probiotic in the gastrointestinal tract of the primate, conferring the benefits of probiotics in supporting and restoring gastrointestinal health. The targeted audience is veterinarians, technicians, husbandry staff, and primate vendors.

This Technical Trade Presentation is sponsored in part by Bio-Serv.