Thursday Morning


W-20 Teaching Monkeys to Cooperate with Restraint: Using Positive Reinforcement Training and Temperament Testing Methods
(8-hour workshop continued from Wednesday 1:00 PM)
8:00 AM - 12:00 PM/Room: 10B
Leader: Jaine E Perlman
Faculty: Mollie A Bloomsmith, Kris Coleman, Jennifer L McMillan
Facilitator: Mark J Prescott
See Wednesday 1:00 PM for workshop description.

This Workshop is sponsored in part by Hybex Innovations, Lomir Biomedical Inc., NC3Rs, and Unifab Corporation.


Innovations in Zebrafish Policies, Husbandry, and Veterinary Care 
8:00 AM - 10:15 AM/Room: Ballroom F
Leader/Moderator: Monte Matthews 
Facilitator: TBN

The zebrafish (Brachydanio rerio) is an important model animal widely used in basic and biomedical research. The expanding scope and scale of zebrafish science demands a more innovated and sophisticated approach to the development and advancement of the institution’s program for the management of institutional policies, husbandry, and veterinary care. We will provide examples from multiple perspectives on successful programs that manage the husbandry and veterinary care of zebrafish facilities. We will discuss improvements in institutional policies and responsibilities for oversight of husbandry and veterinary care management. We will discuss innovations in animal environment, housing, and management and their impact on veterinary care. We will describe the intertwined husbandry and veterinary care components such as standard husbandry practice development (including environmental enrichment), sources of zebrafish, transportation, quarantine, health monitoring and sentinel programs, anesthesia, analgesia, and euthanasia. We will describe common diseases found in zebrafish and improvements for their treatment and control. Finally, we will discuss the advantages, limitations, and integration of improved environmental monitoring and ante mortem samples for zebrafish quarantine and colony health monitoring.


8:00 Monte MatthewsWelcome and Introduction
8:15 Monte MatthewsDeveloping Imaginative Institutional Policies for Zebrafish
8:35 Christian LawrenceInnovation and Reproducibility of Zebrafish Husbandry Practices
8:55 Kathy SnellBasic Improvements in Zebrafish Veterinary Care Programs
9:15 Michael L KentImprovements in Detection of Common Diseases of Zebrafish
9:35 Marcus J CrimInnovations in Health Monitoring

Integrative Physiology for Improved Translation 

8:00 AM - 10:15 AM/Room: 9B
Leaders: Szczepan W Baran, Brian R Berridge, Natalie A Bratcher
Moderator: Szczepan W Baran
Facilitator: Marcel I Perret-Gentil

There is a growing debate about the usefulness of animal studies in biomedical research and drug development. Some of the challenges in translation from animals to human patients may be from weaknesses in how we conduct those studies. Technological advancements are providing opportunities to expand the data we collect from animals and improve alignment to clinical data and data management. Areas of potential 3Rs impact include environmental monitoring and controls, physiological monitoring, behavioral monitoring, and assessments and data management. Technologies are providing innovative approaches to determining optimal housing conditions, improved animal study reproducibility, physiologic and translationally relevant data collection, and ability to iteratively learn from studies in perpetuity. The overall impact of these capabilities when integrated in meaningful ways could reduce animal use, reduce pain and discomfort, and improve clinical predictivity. The target audience includes those interested in learning about how various technological advances are enabling 3Rs impact through improved reproducibility, physiologic, and translationally relevant data including scientists, technicians, veterinarians, IACUC members, or facility managers.


8:00 Szczepan W BaranWelcome and Introduction
8:05 Szczepan W BaranEmerging and Enabling Technologies with 3Rs Impact
8:15 Brian Berridge

Technology-Enabled Comparative Pathophysiology: Strengthening Our Line of Sight to the Patient

8:40 Natalie A BratcherImproved Decision Making Using an Innovative, Non-Invasive Approach to Modeling Chronic Disease to Early Safety Signals in Rodents
9:05 Joseph P GarnerLeveraging Animal Behavior in Equipment Design: Be as a Reed in the Wind, Not An Engineer with a One-Tool Toolkit
9:30 Paul MakidonApplication of Imaging Technologies and Reverse Translation in Rodents for Improved Modeling and Decision Making
9:55 Szczepan W BaranDiscussion

This Seminar is sponsored in part by North American 3Rs Collaborative (NA3RsC) and Innovation and Quality Consortium 3Rs Leadership Group (IQ 3RsLG).

Research Validity and Reproducibility: Everyday Challenges 
8:00 AM - 10:15 AM/Room: Ballroom D
Leader/Moderator: Julie Watson
Facilitator: Alicia Braxton

Lack of reproducibility in research has been a topic of widespread discussion in recent years. Not surprisingly there is considerable concern when public money is invested in research that cannot be duplicated or that fails to translate into successful therapies. As veterinarians, we are in a unique position to consider the whole animal and its environment in relation to the research questions being asked, and are often called upon to troubleshoot when research doesn’t go according to plan. We will use a case-based approach to describe environmental variables that can affect research. Examples from mice and nonhuman primates will demonstrate the effect of husbandry factors such as feed, water, caging and noise; background strain effects such as blindness, deafness, and immune variation; effects of microbial status and medical interventions; and variation due to social stress, abnormal behavior, personality type, and intelligence. This seminar will be of interest to all those that are involved in biomedical research using animals, including researchers, veterinarians, and husbandry staff.


8:00 Julie WatsonWelcome and Introduction
8:05 Cory BraytonMouse and Microbial Contributors to Research Variability
8:50 Eric HutchinsonWeird Things Animals Do and How They Affect Your Research
9:25 Julie WatsonHow Husbandry Procedures, Infections, and Veterinary Therapies Can Affect Research
9:20 Erin S LeeCavy Out of the Box: Adaptations of Guinea Pig Care in an ABSL-3 Study
9:45 Jori LeszczynskiQuestions and Discussion

Use of Dedicated IVC-Based Solutions for Gnotobiotic Mouse Studies: Validation and Operational Optimization 

8:00 AM - 10:15 AM/Room: 18B
Leader: Stephanie W Fowler, Patrick R Hardy
Moderator: Brian Bilecki
Facilitator: Scott Hoy

A growing population of researchers is interested in increasing their facility's capacity for gnotobiotic studies, or is interested in participating in gnotobiotic research but have little experience. Conducting gnotobiotic studies which require comparing different groups of microbiota is cumbersome or even impossible when isolators are the only housing option available. Housing single-study groups in isolators can be difficult due to limited isolator availability, the need to house gnotoxenic groups separately, room capacity, and cost limitations. Constraints combined with the booming interest for microbiota-related studies require a more innovative approach to housing gnotobiotic rodents. Specifically designed positive-pressure IVC units have been developed as a solution to many of these challenges. Upon implementation it is apparent that the specialized IVCs are not a plug-and-play solution, and success relies upon a cooperative review and customization of laboratory SOPs with educated consultants. Information gained from recent studies conducted with this new generation of dedicated equipment will be reviewed. Critical issues include project and local constraints analysis, integration of gnotobiology activity in the existing facilities, associating working cabinet and a suitable transfer systems and sterilization practices to the dedicated caging system, defining technical options, validation of microbiota/germ-free status/control of microbiota over maintenance over time, dealing with all operational practicalities, and adequate project optimization. Attendees will learn advantages and disadvantages of current gnotobiotic housing methods, how to implement newer housing solutions, and adjusting SOPs to greatly increase study volume in their existing facility, demonstrated by real-world implementations of newer housing methodologies. This presentation is targeted toward the growing population of researchers interested in increasing their facility's capacity for gnotobiotic studies, as well as researchers who are interested in participating in gnotobiotic research but have little experience.


8:00 Stephanie C FowlerWelcome and Introductions
8:15 Stephanie W FowlerOptimizing Cage Change and Handling Procedures in Germ-Free Mice when Using Sealed IVC Housing
8:55 Patrick R HardyFrom Feasibility Studies to Operational Optimization: A Focus on Key Issues
9:35 Brian BileckiUse of Dedicated IVC-Based Solutions for Gnotobiotic Mouse Studies: Validation and Operational Optimization

Special Topic Lectures

Anesthetic Management for Laparoscopic and Thoracoscopic Procedures in Laboratory Animals
11:00 AM - 12:00 PM/Room: Ballroom F
Speaker: Dave Daunt
Moderator: Misty J Williams
Facilitator: TBN

Recently there have been dramatic increases in the number and complexity of minimally invasive surgical procedures in humans. Accordingly, more complex and challenging laparoscopic and thoracoscopic procedures and physician training activities have increased in laboratory animals. This lecture will cover the unique problems and complications associated with the anesthetic management for laparoscopic and thoracoscopic procedures. Methods to monitor and support physiologic functions will be covered in detail. Topics will include the unique physiologic challenges associated with insufflation of the abdomen and thorax. Common complications associated with laparoscopic and thoracoscopic surgery will be discussed. Understanding these principles will provide the basis for appropriate patient support, prevention, and treatment of these complications. Several methods of one-lung ventilation will also be presented for thoracoscopic procedures. This talk is targeted to veterinarians and veterinary technicians working in surgical laboratories that use laboratory animals for laparoscopic and thoracoscopic procedure development and physician training.

Effective Training Throughout Application of Learning Theories 
11:00 AM - 12:00 PM/Room: Ballroom D
Speaker: Lisa M Kelly
Moderator: Natasha J Melfi
Facilitator: TBN

Constructive-developmental theory, proposed by famed Harvard developmental psychologist Robert Kegan, asserts that adults develop their own individualized perception of reality and that this perception is continuously evolving throughout their lifepan. This theory was further expanded to include "orders of conciousness" that are believed to directly impact a person's epistemology, or way of knowing. Based on these theories, adults are often expected to handle situations or issues that are beyond the capacity of their mental development. The difference between this expectation and capacity can result in stress and create barriers to learning. This session will explain constructive-developmental theory and propose ways in which trainers can quickly evaluate the orders of conciousness of their learners in order to create an optional environment for the learner to receive and retain new information. The session will explore theoretical ideas on why learners are often resistant to change and how trainers and those in leadership positions can help them throught tough transitions. It will also explore the necessary components for transformational leadership and learning that can successfully influence culture change. The session is ideal for anyone that would like to develop a deeper understanding of the psychology behind adult learning and ways in which theoretical principles can be applied to create effective training programs or sessions.

National Bio and Agrodefense Facility (NBAF): Confronting Global Threats 
11:00 AM - 12:00 PM/Room: 9B
Speaker: Ron W Trewyn
Moderator: Maggie S Behnke
Facilitator: TBN

The National Bio and Agrodefense Facility (NBAF) is a next-generation biocontainment laboratory currently under construction on the campus of Kansas State University in Manhattan, Kansas. Built and operated by the U.S. Department of Homeland Security (DHS), the mission of the NBAF is to protect U.S. livestock from foreign animal diseases (FADs), safeguard public health and food animal health, and preserve America’s food supply and agricultural economy. FADs on the NBAF research agenda include both animal-only and zoonotic biothreats such as African swine fever, foot and mouth disease, Rift Valley fever, and Ebola virus (BSL-4). Once operational, this BSL-3/BSL-3Ag/BSL-4 facility will replace the obsolete Plum Island Animal Disease Center and it will host the USDA Agricultural Research Service (ARS) and Animal and Plant Health Inspection Service (APHIS) as the primary research and diagnostic tenants. Government, industry, and university FAD collaborations are already growing in Manhattan while NBAF is under construction. Participants will learn the significance and current status of NBAF, how the Kansas State University campus was chosen for this location, and the reasons why former Senate Majority Leader Tom Daschle recently called Kansas State University and the area around NBAF “the Silicon Valley for biodefense.” The target audience includes technicians, veterinarians, researchers, biocontainment specialists, and anyone interested in biosecurity, biothreats, or foreign animal diseases.

This Special Topic Lecture is sponsored in part by American College of Laboratory Animal Medicine (ACLAM)/American Society of Laboratory Animal Practitioners (ASLAP) Program Committee.

Using Advanced Modeling and Simulation to Improve Efficiencies in Laboratory Animal Research Facilities 
11:00 AM - 12:00 PM/Room: 18B
Speakers: Kevin Chriswell, Niranjan S Kulkarni
Moderator: Wayne DeSantis
Facilitator: TBN

The presentation will highlight the advantages of using operations improvement modeling, lean design concepts, and animation to enhance the operations and efficiencies in laboratory animal research (LAR) facility design. Through the addition of simulation and animation, the audience will see the benefits of virtual four dimensional analysis and demonstrations of operations within a LAR facility. Combined with building information modeling, the audience will see how operations improvement modeling can enhance mechanical systems design, energy analysis, CFD analysis, and commissioning and validation efforts. The audience will see the benefits of incorporating operations improvement modeling to enhance the design and improve efficiencies of LAR facilities. The target audience includes research institution and corporate laboratory animal facility administrators, vivarium managers, principal investigators, and research grant facilitators.
This Special Topic Lecture is sponsored in part by CRB.

Thursday Afternoon

Panel Discussions 

Biosafety in the Use of Human-Derived Substances in Animal Research 
12:30 PM - 2:00 PM/Room: 19A
Leader/Moderator: Jason S Villano
Facilitator: Jatinder Gulani
Panelist: Stephen Felt, Todd A Jackson, Vanessa B Jensen, Cheryl Perkins, Jason S Villano

The Occupational Health and Safety Administration’s Bloodborne Pathogens standard (29 CFR 1910.1030) prescribes safeguards to protect workers against the health hazards caused by bloodborne pathogens. The standard places requirements on employers whose workers can be reasonably anticipated to contact blood or other potentially infectious materials. This has direct impact on the administration of human-derived substances (HDS) such as cell lines and patient-derived xenografts to animals. This panel will review biosafety concerns related to the use of HDS in animal research, the requirements and expectations of regulatory and accrediting bodies, and approaches, including risk assessment, made by representative institutions in addressing biosafety. Main discussion points will include appropriate containment animal housing and other additional safety procedures. This panel discussion is appropriate for all attendees, including animal care and use program directors, veterinarians, IACUC members, compliance and biosafety officers, and animal care staff.

Getting a Positive Result in a Sentinel Mouse: What to Do after the Diagnosis 
12:30 PM - 2:00 PM/Room: 12B
Leader/Moderator: James O Marx
Facilitator: Kristin L Gardiner
Panelist: Susan R Compton, James O Marx, Abigail Smith

Laboratory animal medicine has devoted a great deal of time and resources to the detection of adventitial organisms in mouse biomedical research facilities. This is important for both the production of high-quality research results and animal welfare. But the detection of the organism is only the first step. Ultimately, eradication of the agent and prevention of future outbreaks is required. Our group recently performed a survey of 63 institutions and found that there is a great deal of variation between institutions in the steps following the identification of an organism. This open, interactive discussion will cover the next steps in the eradication process, including confirmation of an outbreak, isolation procedures, treatment, and confirmation of complete eradication. A series of guided discussions of scenarios will be discussed with the audience, addressing the response to viral, bacterial, and parasitic outbreaks diagnosed by serology, PCR, and/or microscopic examination. Our goal is to provide information to the audience about options in addressing outbreaks of adventitial agents in mouse vivaria. The target audience is individuals involved in quality assurance programs, veterinarians, including laboratory animal medicine residents, and individuals involved in mouse husbandry.

Managing Risk Associated with Outsourcing Animal Work through Effective Diligence 
12:30 PM - 2:00 PM/Room: 17B
Leader/Moderator: Linda K Fritz
Facilitator: TBN
Panelist: Natalie A Bratcher, Wendy J Underwood, Gina P Wilkerson

Pharmaceutical companies routinely place animal work at contract research organizations (CROs), universities, biotech companies, and governmental organizations. This work may involve collaborative research projects, single-study contracts, or master service agreements. A number of risks should be considered when placing work at a third party. Items to consider include risks associated with the animal work, including the care and welfare program at the contract or collaborative institution, and study-related risks associated with the work. This panel will share their risk mitigation strategies including diligence prior to work being placed and the following oversight plans. The learning objectives of the panel are to understand the risks associated with various types of externalized contracts or collaborations, to share diligence strategies for evaluations, to consider third-party animal welfare care and use programs, to study related risks, to discuss strategies for ongoing monitoring and site visits, to share IT solutions for managing assessment of outsourced work and collection of metrics, and to discuss the impact of pharma expectations on the third parties with the participants. Target audience includes contract or scientific staff responsible for placing animal work, third-party personnel responsible for compliance with pharma expectations, and those interested in risk management.

Scheduling Models and the Implementation of a Task-Driven Staff Assignment 
12:30 PM - 2:00 PM/Room: 16A
Leader: Tasha M Thomas
Moderator: Ann L Murray
Facilitator: TBN
Panelist: David W Brammer, Tasha M Thomas, Yehia Wafa

In order to maximize productivity and operate efficiently, there should be a clear course of action on staff responsibilities and specific work duties for each team member. However, effective planning is time consuming and complex when considering the wide variety of activities and the proper resources necessary to support them. This includes materials, functioning equipment, and staff. In addition, the work becomes more complex when considering the live animals and the varied species staff may be required to work with. The implementation of a sound schedule impacts the work flow, staff satisfaction, and overall success of your laboratory animal research program. The presenters will provide an overview of several scheduling models and ways to optimize work efficiency through staff scheduling. The presenters will also provide an overview of the pros and cons of implementation, and a specific review of a task-driven scheduling model. In addition, special emphasis will be given to the task-oriented scheduling model and how it is used at two lean management facilities. The panel discussion will cover an introduction to lean management principles and discuss how lean management can be used in staff scheduling. The panel discussion will also include an overview of the room assignment scheduling model, the team captain area approach scheduling model and the task-oriented scheduling model. The panel discussion will allow participants to discuss the advantages and disadvantages of each system and the methods of implementing each system.