Tuesday Morning


W-01 CMAR Prep Course (continued from Monday 8:00 AM)

8:00 AM - 12:00 PM/Room: 202B
Leader/Faculty: Camellia M Symonowicz, Diana P Baumann
Facilitator: TBN

See Monday 8:00 AM for description.

This Workshop is sponsored in part by Stowers Institute.

W-08 Operation of a Gnotobiotic Mouse Husbandry and Technical Program “Gnoto 201”
8:00 AM - 12:00 PM/Room: 210A
Leader: Julia R Krout
Faculty: Joana Bom, Stephanie M Cormier, Andrea Crawford, Allison R Rogala, Alton G Swennes, Betty R Theriault
Facilitator: Erin Evers
Workshop Fee: $140 Workshop Limit: 50

This workshop is designed to complement the Isolators 101 workshop. It will help participants who have a basic understanding of flexible film isolators gain additional insights into practical aspects of their use. The workshop will cover isolator validation methods, husbandry and supply considerations, microbiological testing, and recordkeeping. This workshop targets anyone in the laboratory animal field interested in learning more about working in different isolators and caging systems, gnotobiotic facility management, and operation. The workshop will have an emphasis on operations that is geared to husbandry technicians, managers, veterinary technicians, veterinarians, research scientists, vendors, and more. This workshop integrates well with the popular and long-standing Isolators 101 workshop and the Development of a Gnotobiotic Mouse Husbandry and Technical Program “Gnoto 301” workshop. The information presented in this workshop is distinct from that presented in the Isolators 101 workshop and Development of a Gnotobiotic Mouse Husbandry and Technical Program “Gnoto 301” workshop. Suggestions from last year’s inaugural workshop emphasized more time for the two main topics of the 2015 workshop, Development and Operation of a Gnotobiotic Mouse Husbandry and Technical Program. Therefore, operations topics are now featured in the Gnoto 201 workshop and development topics are explored in the “Gnoto 301” workshop. Presenters will guide the workshop attendees through operational considerations using hands on techniques and real-life scenarios. The workshop will also include advanced technical procedures.

This Workshop is sponsored in part by Charles River, Class Biological Clean, and Tecniplast.

W-09 Perioperative Monitoring during Anesthesia Made Easy
8:00 AM - 12:00 PM/Room: 201B
Leader: Cholawat Pacharinsak
Faculty: Charles Tyler Long
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 30

Perioperative anesthetic monitoring is an important part of anesthesia in animal research. The main goal of anesthetic monitoring is to alert anesthetists of potential complications before they become irreversible. If anesthetists are not familiar with anesthetic monitoring parameters and their interpretation, this can be a daunting task for them. The workshop will help attendees become familiar with perioperative anesthetic monitoring. This workshop will focus on understanding commonly used anesthetic monitoring equipment (ECG, blood pressure, %SpO2, ETCO2, and body temperature) and their importance; from indications and implementations, to interpretations during large animal anesthesia. This dry lab workshop is appropriate for veterinarians, laboratory animal technicians, scientists, laboratory managers, and IACUC members who are not familiar with anesthetic monitoring techniques.

This Workshop is sponsored in part by VetEquip.

W-10 Writing Standard Operating Procedures that Work
8:00 AM - 12:00 PM/Room: 209A
Leader: Ann L Murray
Faculty: Norm P Moreau, Deborah A Wenger
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 50

Standards Operating Procedures (SOPs) are a fundamental prerequisite of any quality management system in research and yet organizations frequently neglect their importance, development, use and management, thereby putting them at risk for noncompliance. The best SOP is one that accurately transfers the relevant information and facilitates compliance with reading and using the SOP. This interactive workshop is designed for those who are responsible for developing and maintaining SOPs at GLP/Non GLP animal care facilities or laboratories. Participants will learn the essential components for writing effective, user-friendly SOPs starting with the critical technique of process mapping. Learners will engage in activities for drafting SOPs and will have the opportunity to bring an SOP for evaluation and feedback. Each participant will receive a handbook of writing guidelines, SOP templates, and sample SOPs.


Cotton Rat: An Alternative Model for Rats and Mice

8:00 AM - 10:15 AM/Room: Ballroom B
Leader/Moderator: Krishnan Kolappaswamy
Facilitator: TBN

The cotton rat serves as an excellent alternative model for pathogens that do not infect conventional mouse and rat models. It presents both challenges and opportunities when used in a research setting. Although it is a rodent, the cotton rat is a USDA-regulated species which necessitates documentation, record keeping, and reporting not required when using rats and mice. Moreover, cotton rats are uniquely susceptible to a wide range of human respiratory viruses. Most notable is the use of cotton rats for respiratory syncytial virus (RSV) research in the development of a prophylactic monoclonal antibody, pre-clinical testing of vaccines, and of antiviral agents. In addition, cotton rats are also susceptible to measles virus, human parainfluenza virus, human metapneumovirus, and influenza virus, and thus have been utilized for comparative studies. These studies are supported by the advent of ever-increasing genetic information, the use of sophisticated equipment to analyze respiratory function during infection, and the expanding number of methods and reagents available to analyze the cotton rat immune response. The housing, husbandry, training on technical procedures, enrichment, and health surveillance all provide unique challenges. This presentation will include the challenges and options for solutions that can be implemented, as well as recent updates on research uses of cotton rats. The target audience includes veterinarians, investigators, facility managers, and technical personnel.


8:00 Krishnan KolappaswamyWelcome, Introductions, and History of Cotton Rats
8:15 Kim A FroeschlChallenges in Using Cotton Rats
8:45 Stefan NiewieskResearch Use Updates on Cotton Rats
9:30 Mandy J HornHusbandry and Management of Cotton Rats

This Seminar is sponsored in part by American College of Laboratory Animal Medicine/American Society of Laboratory Animal Practitioners (ACLAM/ASLAP) Program Committees.

Get the Dirt on Rack Testing: New Data Examining the Effectiveness of Environmental Monitoring for Animal Health Surveillance
8:00 AM - 10:15 AM/Room: Ballroom C
Leader/Moderator: Lela K Riley
Facilitator: TBN

The role of environmental monitoring in animal health surveillance programs has been the subject of a great deal of discussion and confusion. Samples collected for environmental monitoring may include plenum or port swabs, filters or pre-filters, or commercial products designed to capture exhaust air debris. Some have suggested that environmental monitoring can replace sentinels for health surveillance. Others believe that reliance on environmental monitoring may result in failure to detect infectious agents, compromising the biosecurity of research animals. Still others express confusion about the value of environmental monitoring for their facility and concern about the risk associated with importing animals from an institution that relies strictly on environmental monitoring for health surveillance. The goal of this seminar is to present new evidenced-based studies, both experimental studies and real-life scenarios, regarding the effectiveness of environmental monitoring. Participants will learn about the differences among ventilated caging systems and their utility for environmental monitoring. They will also learn about the effectiveness of various environmental sampling methods and sites for detection of rodent pathogens. The target audience includes veterinarians, facility managers, and technical personnel who manage health monitoring programs.


8:00 Lela K RileyWelcome and Introductions
8:05 Beth A BauerHousing Matters
8:15 Cynthia L Besch-WillifordClosed Systems: They’re Called This for a Reason
8:35 Robert S LivingstonOpen Systems: Dust in the Wind
9:05 Mary Wight-CarterPinworms: A Real-life Scenario Using Environmental Monitoring
9:25 Chris A ManuelCorynebacterium bovis: A Real-Life Scenario Using Environmental Monitoring
9:45 Mathias LeblancMurine Norovirus: A Real-Life Scenario Using Environmental Monitoring
10:05 Lela K Riley Summary

IACUC Fundamentals: Myths and Facts

8:00 AM - 10:15 AM/Room: Ballroom A
Leader: Marcy A Brown
Moderator: Mary Jo Shepherd
Facilitator: TBN

Four Certified Professional IACUC Administrators (CPIAs), with experience working with IACUCs at AAALAC-accredited/PHS-assured/USDA-registered facilities, will discuss and review IACUC functions with an emphasis on understanding committee processes. Discussions will include what is and is not required for the IACUC protocol review process and semiannual program reviews and inspections. Investigation of reports of noncompliance, submission of annual reports, documentation of meeting activities, provision of training, and conduct of post approval monitoring will also be included. This will be a highly interactive session with the intent of engaging audience members in open dialogue. This diverse seminar is ideal for investigative staff that interacts with their local IACUC office, husbandry staff as they prepare for and assist with facility inspections, and IACUC members/staff who simply need reassurance that they are on the right track.


8:00 Marcia A BrownIACUC Fundamentals: Facts and Myths
8:15 Deb FrolicherProtocol Review and Documentation
8:35 Molly GreeneQualifications, Training, and Occupational Health
9:00 Mary Jo ShepherdSemiannual Inspections and Program Review
9:20 Marcia A BrownPost Approval Monitoring and Inspection Readiness
9:40 All Questions and Answers

What’s a Rodent to Drink?
8:00 AM - 10:15 AM/Room: 217C
Leader: Judy M Hickman-Davis
Moderator: Stephanie D Lewis
Facilitator: Dana M LeMoine

The choice of water delivery system for vivaria is determined by species, facility, and type of water available. Water for rodent species may be supplied to cages by water bottles, plastic pouches, or automatic water systems. Ventilated rodent racks may support automatic water systems with water valves permanently attached to the rack, removable valves attached to the rack, or valves attached permanently to the inside of the cage. Water valves permanently attached to the inside of the cage provide challenges for sanitation, requiring more attention for removal of bedding that may become caught around the inverted stem. Water valves that remain affixed to the rack provide the opportunity for cross contamination of cages if cage locations are inadvertently switched. Practices for storage, distribution, and use of water bottles or bags, as well as maintenance and cleaning of lixit valves and water lines vary widely between institutions. Standard operating procedures should be developed to meet the individual barrier and equipment conditions and should be designed to minimize cost, maximize function, minimize cross contamination, and facilitate research. This session will review the types of water available for rodent water systems, including city water, filtered water, reverse osmosis, UV treated and the quality assurance necessary to maintain these systems. The use of plastic bags and water bottles will be reviewed with focus on the pros and cons of each system, distribution, use at cage change out, investigator training, and quality assurance. Automatic water lines will be discussed with a focus on quality assurance for flushing and maintenance of water lines, different types of lixit systems, investigator training, cage flooding, and cleaning/ disinfection of the lixit valve. The cost differential involved in using plastic pouches, water bottles and automatic watering systems for maintenance of rodent colonies will be discussed. The target audience is anyone involved in the day to day maintenance of rodent colonies, animal care program directors, veterinarians, and facility managers.


8:00 Judy M Hickman-DavisWelcome and Introductions
8:10 Stephanie LewisDrinking Water Sources and Available Water Treatments for Laboratory Rodents
8:40 Samuel C CartnerUtilization of Plastic Water Pouches for Rodent Housing Systems
9:10 Judy M Hickman-DavisAutomatic Watering Systems, Quality Assurance for Water Lines, and Lixit Systems
9:40 Cecile BaccanaleThe Cost of Rodent Water


8:00 AM–10:45 AM

Platform Session abstracts will be available on www.aalas.org in July and will be included in the National Meeting Final Program.


Charles River Ethics and Animal Welfare Lecture: A Good Life for Lab Animals

11:00 AM - 12:00 PM/Room: Ballroom A
Speaker: Dan Weary
Moderator: Marilyn J Brown
Facilitator: TBN

Here is my dream: we build a world in which I can take pride in my animals and the care they receive, that when I meet someone I introduce myself as someone who cares for animals used in research, and that I invite anyone who is interested to come and meet the animals I work with. In this talk I will describe different ways of thinking about animal welfare, ranging from animal suffering to the good life, and describe approaches from our research to studying these issues scientifically. I will share our most recent thinking on how we can assess pain and distress in animals, and how to meaningfully distinguish these states from suffering. I will discuss issues of agency and how this relates to welfare, and I will give examples of how housing and management can change the lives of the animals and how we relate to them. I will also describe what work on human attitudes shows about the importance of transparency and how current practices may have the counter-productive effect of increasing distrust in the science and accentuating welfare concerns. Finally, I will leave the audience with an example of a research facility always open to the public, in which researchers and animal care workers introduce their animals with pride, and for which both the animals and the people who care for them can be considered to have a good life. Participants will be introduced to different ideas for how to assess quality of life in research animals, and to scientific approaches to assessing different aspects of animal welfare. The audience will also be introduced to methods of assessing the attitudes of people to the use of animals in research, and how these are affected by transparency. Target audience is all attendees at the national meeting.

This Special Topic Lecture is sponsored in part by Charles River.

Organized Veterinary Medicine and Biomedical Research: Lead, Leverage, and Learn
11:00 AM - 12:00 PM/Room: Ballroom C
Speaker: Stacy L Pritt
Moderator: Kathryn L Cavanaugh
Facilitator: Meredith R Brown

The world of organized veterinary medicine encompasses multiple associations. Beyond hosting conferences and providing continuing education, these associations are shaping the future of veterinary medicine in the United States by establishing guidelines on animal welfare, establishing legislative priorities, and developing the next generation of veterinary leaders. Laboratory animal medicine and biomedical research are important sectors of veterinary medicine that must be heard within veterinary associations. The American Society of Laboratory Animal Practitioners (ASLAP) has a formal relationship with the American Veterinary Medical Association (AVMA), but there are other opportunities outside of research that can be leveraged to advance individual veterinarians, veterinary technicians, and the entire field of biomedical research. This session will explore opportunities for involvement for all individuals interested in advancing the veterinary profession, advocating for biomedical research, and developing leadership skills.

This Special Topic Lecture is sponsored in part by Women’s Veterinary Leadership Development Initiative, American Veterinary Medical Association (AVMA), American Society of Laboratory Animal Practitioners Program, and American College of Lab Animal Medicine (ASLAP/ACLAM).

Spineless not Heartless: A Scientific Look at Pain in Invertebrates and the Ethics of Uncertainty in Animal Research
11:00 AM - 12:00 PM/Room: 217C
Speaker: Brad Ahrens
Moderator: Trinka W Adamson
Facilitator: TBN

Invertebrates have been widely used in research for over a century. To many, they represent a popular replacement for vertebrate species under the assumption that they are insentient, or at least less sentient than vertebrate animals. Although most invertebrates can perceive and react to something noxious or harmful, without cognition, they are by definition, incapable of feeling pain. Is this contention true? Do invertebrates experience pain like humans and other animals? Can these animals suffer as a result of our experiments? This lecture will present a variety of current and classic research demonstrating evidence of cognition (or lack thereof) in various invertebrate species that we generally assume to be incapable of feeling pain. This will be contrasted with what we typically accept as pain in ourselves and other vertebrates. Did you know that jumping spiders plan complex attack strategies, bees become depressed and turn down treats after their hive is attacked, isolation-reared cockroaches have trouble finding a girlfriend later in life, and snails will repeatedly press a lever for electrical sexual stimulation? Although these findings, among other interesting experiments to be presented in this talk, do not prove that these animals are sentient, they caution us that we may have more reasons to accept, rather than reject, the assertion. This topic will be approached from a practical, rather than a philosophical point of view. Biological evidence, from both literature and personal research, will be presented and explored as to how this evidence supports or refutes the notion that these animals are “sentient enough” to experience pain. The potential role of IACUCs and oversight agencies, as well as current regulations surrounding invertebrate research, will also be discussed and contrasted with that of other countries. Finally, we will objectively explore the “ethics of uncertainty” in both vertebrate and invertebrate research, presenting both the ethical and economic cost of guarding against the possibility that invertebrates feel pain. If we use anesthetics or analgesics and it turns out that they don’t experience pain, the material cost of our mistake is relatively low. However, if we choose not to take these precautions and it turns out that the animals were in agony, then the moral cost of our mistake is, to some, quite high. This presentation is intended for people of all backgrounds as a fun and novel discussion of the growing wealth of interesting research that may just inspire some of us to re-evaluate our feelings and maybe even our regulations about invertebrates and their treatment as laboratory animals.

Wallace P Rowe Lecture
11:00 AM - 12:00 PM/Room: Ballroom B
Speaker: TBN
Moderator: Dondrae J Coble
Facilitator: TBN

Speaker and description will be available after the Award Selection Committee selects the Bhatt Award Recipient in June 2016.

This Special Topic Lecture is sponsored in part by Committee for Laboratory Animal Training and Research (CLATR).


Making It Stick: Tips and Tools to Enhance Learner Retention

12:30 PM - 2:00 PM/Room: 207A
Leader: Lisa M Kelly
Moderator: Tiffany L Whitcomb
Facilitator: Rebeccah G Hunter
Panelists: Tom T Chatkupt, Kim A Froeschl, Lisa M Kelly and Lisa K Secrest

Effective training programs are an essential component of high-quality animal care and must be dynamic, interactive, and evolving. Animal care programs are increasingly focused on performance-based training goals to meet the objectives of regulators and accrediting bodies. Additionally, scientific advances in this field have led to an even greater demand for highly trained staff. This discussion will bring together a range of instructional experts from academia, commercial, and pharmaceutical industries that will provide best-practice advice on how to create training that maximizes learner retention. They will share their tips, tools, and techniques for the development of instruction that is learner-focused and goal-oriented. The panel will discuss how to apply creative tools to a variety of instructional modalities (for example, online, didactic, and hands-on.) The audience will be encouraged to ask questions and brainstorm on solutions to their own training challenges.

This Panel Discussion is sponsored in part by Laboratory Animal Welfare Training Exchange (LAWTE).

Nuts About Welfare: Social Housing and Enrichment Strategies
12:30 PM - 2:00 PM/Room: 213C
Leader: Stephanie D Lewis
Moderator: Dana M LeMoine
Facilitator: Jacquie Stewart
Panelists: Valerie K Bergdall, Justin D Kieffer, Stephanie D Lewis, Amanda E Sparks

Refinement of housing and husbandry practices in laboratory animal medicine is an ongoing endeavor. Common strategies applied in the field often include the provision of social companions, modification of their physical environment, or novel types of and/or presentation of food. SOPs should be developed to address social housing and enrichment provision and should be designed to minimize cost and facilitate research while meeting the needs of the animals. Designing a program that is not overly complex and yet still provides novelty via rotation can be difficult, especially at a large institution. This process becomes even more challenging when nonstandard species are housed, which require special consideration. As welfare standards continue to change for animals housed in agriculture settings, refinement of environmental enrichment and social housing paradigms in this context should be addressed. The institutional cost of developing and maintaining an enrichment program and how to budget for expenditures such as facility modification to accommodate social housing of certain species will also be discussed. This session will address social housing and enrichment for a myriad of species in different housing situations at a large institution. However, the strategies provided are applicable and easily modified to institutions of any size. Designing a simple, visual, rotational enrichment program will be discussed for standard laboratory animals, as well as how to develop a social compatibility SOP for social housing of species. Additionally, the challenges of social housing and provision of enrichment to unique species housed in a biomedical setting will be addressed. Implementation, refinement and welfare considerations for teaching and research animals housed in an agriculture setting will also be examined. The cost of implementing and maintaining an enrichment program as well as financial considerations to modify facilities to accommodate social housing will also be analyzed. The session is directed toward animal care program directors, veterinarians, facility managers/supervisors, laboratory animal health technicians, and enrichment coordinators and individuals involved in daily care of animals.

Reaching the Roots: Biomedical Research Awareness Day Builds Awareness of Animal-Based Research
12:30 PM - 2:00 PM/Room: 213B
Leader/Moderator: Paula A Clifford
Facilitator: Adam Werts
Panelists: Cindy A Buckmaster, Logan K France, Jaclyn R Steinbach, Veterinary Student (TBN)

Whatever their area of practice, it is important that all veterinarians understand the critical role of laboratory animals in the quest for treatments and cures. Conveying this awareness to students during their veterinary education establishes a foundation of knowledge and support for biomedical research and increases awareness of laboratory animal medicine as a possible career choice. Biomedical Research Awareness Day (BRAD) was created this year as a project by an AMP/Hayre Fellow who received a resounding response by most of America’s veterinary schools. The first BRAD was held the third week in April; twenty veterinary schools across the country participated in the observance to provide more information about animal-based research and to honor the contribution of laboratory animals to medical progress. The intent is for BRAD to become an annual event, involving an increasing number of faculty and students each year. Lectures, interactive displays, giveaways, guest speakers, and other activities at BRAD observances provide opportunities at each school to begin a dialogue on the ongoing necessity for animal research, and the potential for careers in laboratory animal medicine. This panel and participants will discuss various methods of outreach and education that were employed in the inaugural BRAD, including the use of social media, the planning, implementation, impact of BRAD, and the importance of garnering the support of new generations of veterinarians. Attendees will have the opportunity to become involved in expanding BRAD, developing awareness projects and events that will support the animals involved in biomedical research. Outreach and education are the pillars of BRAD. Thus, it is crucial to expand the input and support of those in the field if we are to maximize the impact of this program. We look forward to a vigorous discussion among veterinarians, students, technicians, scientists, educators, and others on how to shape BRAD based on what we learned in its first year, how to spot and overcome potential obstacles, and how to bring more veterinary schools and research institutions aboard so BRAD might be expanded in successive years.

Through the Looking Glass: Acknowledging the Impact of Privilege and Intersectionality in Laboratory Animal Science
12:30 PM - 2:00 PM/Room: 207D
Leader: Michael K Fink
Moderator: Sheba R Churchill
Facilitator: Angela P King-Herbert
Panelist: William A Hill, Craig L Franklin, Mildred M Randolph

As laboratory animal science professionals we interact with research and animal care staff from increasingly diverse and varied backgrounds. In spite of the benefits that a diverse workforce provides our field, as a profession we have been slow to recognize and adequately discuss how the topics of privilege and intersectionality impact the individuals supporting laboratory animal science. Privilege is a pervasive social structure that confers advantages and unearned benefits to individuals within specific social groups. The traditional example is Caucasian males, who with all other things being equal, have an inherent advantage over individuals of other demographics simply for being born a white male. Privilege is now thought of as being multi-faceted, with individual forms of privilege (sex, age, race, sexuality, religion, etc.) encompassing perceived or actual differences between individuals. Intersectionality recognizes that people possess multiple identities constructed from social interactions and social structures, including privilege, and that individuals can experience multiple types of disadvantage due to the combination of these identities. Recognizing and acknowledging forms of privilege and how they influence our interactions with those around us is vitally important to challenging systematic oppression. In this panel discussion, a cohort of diverse laboratory animal science professionals will engage meeting attendees in a safe space, discuss the concepts of privilege and intersectionality, offer perspectives gleaned from personal experiences with privilege, and offer suggestions for promoting the recognition of privilege as a first step towards dismantling this overlooked form of oppression in our field. This panel discussion is appropriate for all meeting attendees and will encourage constructive conversation and audience participation on the topics of privilege and intersectionality within the field of laboratory animal science. Attendees will learn that many of their peers are disadvantaged in ways not previously realized and that both privilege and intersectionality should be taken into account when addressing efforts for positive change in the workplace.


W-11 Animal Facility Design, Processes, Decisions, and Technology

1:00 PM - 5:00 PM/Room: 201B
Leader: Mark A Corey
Faculty: Mark B Gold, Laura Halverson, Lauri Kempfer, Kathleen L McGimpsey, Blythe M Vogt
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 50

This session will benefit those involved with animal facility design and operations by describing the processes, decisions, and technologies involved in the design, construction, and management of animal facilities. The workshop will begin with a discussion of the facility design process, who should be involved, the level of effort by stakeholders, milestone decisions to be reached, and anticipated durations of the various phases of the process. Next, current trends in the industry will be explored through discussions about planning, interior construction, and finishes. Next, we will present a case study about creative facility solutions for housing large animals, including an open discussion comparing EU and AWA regulations for these kinds of facilities. Lastly, we will have a presentation about mechanical, electrical, and piping design and operations. The discussion will focus on the risks associated with animal welfare, loss of research and facility resiliency, and how engineering decisions affect each of these parameters. These lessons learned will help enable participants to make more informed decisions as they develop and operate their own facilities. Finally, planning energy- and resource-efficient facilities is no longer a trend or an option, but rather an integral driver in facility planning. We will discuss sustainability strategies regarding energy and water that are being effectively implemented in animal facilities and the long-term benefits derived from each.

W-12 Development of a Gnotobiotic Mouse Husbandry and Technical Program “Gnoto 301”
1:00 PM - 5:00 PM/Room: 210A
Leader: Julia R Krout
Faculty: Joana Bom, Stephanie M Cormier, Andrea Crawford, Allison R Rogala, Alton G Swennes, Betty R Theriault
Facilitator: Erin Severs
Workshop Fee: $140 Workshop Limit: 50

This workshop is designed to complement the Isolators 101 and Gnoto 201 workshops. It will help participants who have a moderate understanding of flexible film isolators gain insights in developing a gnotobiotic husbandry and technical program. The workshop will cover finding space, funding, choosing equipment, cost recovery and fee structure, staffing, and training. This workshop targets anyone in the laboratory animal field interested in learning more about gnotobiotics program facility design, management, and operation with emphasis on design and management. This includes managers, veterinarians, research scientists, vendors, and more. This workshop is distinct from, but integrates well with the popular and long-standing Isolators 101 workshop and Operation of a Gnotobiotic Mouse Husbandry and Technical Program “Gnoto 201” workshop. Suggestions from last year’s inaugural workshop emphasized more time for the two main topics of the 2015 workshop Development and Operation of a Gnotobiotic Mouse Husbandry and Technical Program. Therefore, operations topics are now featured in the Gnoto 201 workshop and development topics are explored in the Gnoto 301 workshop.

This Workshop is sponsored in part by Taconic, Class Biological Clean, and Allentown.

W-13 Sew You Learned to Tie a Knot, But Did You?
1:00 PM - 5:00 PM/Room: 209A
Leaders: Marcel I Perret-Gentil, Szczepan W Baran
Faculty: John J Bogdanske, Jared R Hammer, Mitchell M Moore, Luis M Zorrilla
Facilitator: TBN
Workshop Fee: $140 Workshop Limit: 35

Rough tissue handling and prolonged tissue exposure to room air increases surgical trauma. This in turn affects post-operative recovery and data outcome. During this workshop, participants will learn commonly used suture and knot-tying techniques, among other things. The workshop will focus on appropriate hand-eye coordination to quickly improve suturing skills. Participants will have an opportunity to learn techniques that will lead to gentle handling while completing suturing tasks at incredible speeds. A variety of training tools, including a state-of-the-art inanimate model to practice suturing, will be introduced. These suturing tools aid in the development and instruction of basic and advanced suturing skills. These affordable and portable products are perfect for beginners just learning or experts who need to further refine complex suturing techniques. In this interactive workshop, participants will first be provided with an overview of basic suturing principles. Participants will have an opportunity to practice and improve their suturing skills at each station. Common errors and complications will be introduced and addressed. This workshop is designed for individuals who have minimal or no suturing skills, but is also a great opportunity to upgrade skills for those with considerable experience.

W-14 Teaching Monkeys to Cooperate with Re straint: Using Positive Reinforcement Training and Temperament Testing Methods
(8-hour workshop, continued Wednesday 8:00 AM)
1:00 PM - 5:00 PM/Room: 202B
Leader: Jaine E Perlman
Faculty: Mollie A Bloomsmith, Kris Coleman, Jennifer L McMillan
Facilitator: Mark Prescott
Workshop Fee: $250 Workshop Limit: 50

The workshop includes 8-hours of instruction on using positive reinforcement training (PRT) to teach monkeys to cooperate with various restraint procedures, and provide information about utilizing temperament testing to assist in the selection of subjects and planning for their training. Participants will learn approaches to training laboratory primates to cooperate with restraint for sample collection (e.g., blood, vaginal fluids) and for chair restraint. PRT is an important refinement in the care of nonhuman primates and is an effective means of improving their welfare. However, animals respond differently to restraint and measuring temperament provides insight into how individuals might respond to these procedures, allowing for individualized and more effective training plans. Workshop goals: 1) introduce participants to animal training terminology and techniques; 2) teach PRT techniques as they apply to restraint procedures, such as the use of the cage squeeze back mechanism and chair restraint; 3) teach methods to assess and quantify temperament in monkeys and to use this information to develop individualized training plans. Participants will learn how to establish a strong foundation for successful restraint training using PRT techniques, and how to incorporate alternative techniques such as negative reinforcement to meet research timelines. They will learn to identify monkeys who are engaged in the training process and how to increase the involvement of monkeys who seem uninterested in training. Participants will learn how to shape behavior and apply desensitization techniques, how to maintain trained behaviors over time, and how to transfer trained behaviors among multiple staff members. Participants will learn how temperament can impact training approaches and the anticipated timelines for training to cooperate with restraint. Understanding the intersection of individual differences in temperament and animal training will aid in the design of more efficient animal training programs. This workshop is designed for those experienced in working with primates including behavior specialists, animal caregivers, research technicians, animal managers, veterinarians, and principal investigators.

This Workshop is sponsored in part by NC3R, Lomir Biomedical, Crist Instrument, and Unifab Corporation.


50 Years of the Animal Welfare Act: Protecting, Promoting, and Advancing the Welfare of Animals in Research

2:45 PM - 5:00 PM/Room: Ballroom B
Leaders: William S Stokes, Krishnan Kolappaswamy
Moderator: Krishnan Kolappaswamy
Facilitator: Sheryl J Wildt

The Animal Welfare Act (Public Law 89-544) was signed into law on August 24, 1966, becoming the first federal law protecting the welfare of laboratory animals in the United States. The act has been amended several times during the past 50 years to further define USDA’s jurisdiction over the care, use, and management of laboratory animals, including requirements for IACUCs to oversee animal care and use at registered research facilities. This session will review the history of the AWA and its amendments and the evolvement of USDA administrative oversight leading to the current animal care organization within the Animal and Plant Health Inspection Service (APHIS). Trends in research facility compliance with AWA regulations will be discussed, with a focus on current inspection processes and the most frequent citations in research facilities. Trends in the number and species of animals used in research and their pain categories will be presented, including a review of the rationale for and numbers and species of animals that currently experience unrelieved pain and distress. Finally, federal audits of APHIS oversight of research facilities and AWA regulations and the impact those audits have had on research along with subsequent audit recommendations will be discussed.


2:45 William S StokesWelcome and Introduction
2:55 Robert M GibbensHistory of the Animal Welfare Act and USDA Animal Care
3:25 William S StokesTrends in Research Facility Compliance with the Animal Welfare Act
3:55 Nicolette A PetervaryTrends in Laboratory Animal Pain and Distress
:25 Betty GoldentyerFederal AWA Audits and Impact on Research

This Seminar is sponsored in part by American College of Laboratory Animal Medicine/American Society of Laboratory Animal Practitioners (ACLAM/ASLAP) Program Committees.

Advancements in Research Cephalopod Care and Welfare
2:45 PM - 5:00 PM/Room: Ballroom A
Leader: Graziano Fiorito
Moderator: Viola Galligioni
Facilitator: TBN

Beginning in 2013, Directive 2010/63/EU required inclusion of cephalopods as the sole invertebrates on the list of laboratory animals utilized for scientific purposes, similarly to what is already established for vertebrates. This has a huge impact on cephalopod research in EU countries, but it also represents the occasion to set up best practices for their care and welfare. In addition, the ARRIVE guidelines, which many scientific journals, funding bodies, universities, and societies have adopted, address the inclusion of cephalopods. The EU Directive affects not only research carried out in EU, but many other countries worldwide. During this seminar we will provide some examples of how we are addressing the welfare of cephalopods in research. Nociception and pain perception and behavioral and physiological cases will be discussed to illustrate how research with cephalopods requires attention, guidelines, and trainings opportunities to comply with the directive. The target audience includes IACUC administrators and committee members, vets, facility managers, researchers, and technicians involved in regulation and those working directly or indirectly with cephalopods.


2:45 Graziano FioritoWelcome and Introduction
3:00 Graziano FioritoWhat’s New in Cephalopod Research in EU (Directive 2010/63/EU) and Current Status of Training Opportunities
3:30 Robyn CrookWelfare Insights from Studies of Pain-Related Sensory Physiology
4:00 Jennifer MatherBehavior and Welfare Considerations
4:30 Viola GalligioniSetting Up Housing and Care

Animal Models in Neurotrauma Research

2:45 PM - 5:00 PM/Room: Ballroom C
Leader: Joseph McCabe
Moderator: David E Bentzel
Facilitator: Michael C Junio

Awareness of the consequences of traumatic brain injury (TBI) for the individual, their families, and national health care systems has become a prominent issue. Concern about youth and professional contact sports, military personnel exposure to accidents and blasts, and hypothesized relationships between early TBI and insidious, late-life dementia have all received attention in the media. Animal models of TBI play a key role in trying to understand the neuropathological consequences of TBI. In this session, investigators will provide an overview of commonly used models that mimic aspects of TBI, from injuries related to violent impact to shock wave injuries from blast. The investigators will describe the rationale for the models and how researchers utilize animals to study neuropathological consequences of TBI. Finally, participants will be exposed to future research directions and challenges. The target audience is veterinarians, veterinary technicians, IACUC members, researchers, and anyone with an interest in animal models of TBI.


2:45 David E BentzelWelcome & Introductions
2:55 Joseph McCabeRodent Modeling of Traumatic Brain Injury (TBI)
3:30 Fabio LeonessaRodent Models of Blast-Related Neurotrauma
4:05 Cameron “Dale” BassModeling Neurotrauma Using Animals: The Large and the Small of It
4:40 AllQuestions and Answers

This Seminar is sponsored in part by American College of Laboratory Animal Medicine/American Society of Laboratory Animal Practitioners (ACLAM/ASLAP) Program Committees.

Utility Costs Rising—Global Warming and the Animal Facility
2:45 PM - 5:00 PM/Room: 217C
Leader: James D Cox, Bruce W Kennedy
Moderator: Bruce W Kennedy
Facilitator: TBN

The notion that natural resources, such as water and fuels for power, are critical to the operation of animal research facilities is very familiar to us. Cagewash areas, for example, use copious amounts of water, steam, natural gas, and electricity. HVAC systems depend upon them for heating, cooling, and humidification. As importantly, we know that such resources are in limited supply and have environmental impact. Parts of the country, like California, suffer from long periods of drought. Fossil fuels used to generate electricity are limited too and when burned contribute to increases of the CO2 footprint. Similar terms like green and sustainability have become global buzzwords, but no less important are the responsibilities at the local level. So, what are lab animal facility managers considering to mitigate these concerns? Without unlimited natural resources, each has a social and economic reason to consider ways to create a more sustainable facility. Conservation is one effective approach. Optimization is another. Several lab animal colleagues have already had to contemplate and experience such challenges, all of which will be shared in this seminar aimed at cagewash staff, facility managers, and especially front office accountants. They will bring insight and practical examples to the discussion of questions such as, where are the important decision-making points. What can cagewash personnel do now for maintenance, quality control, equipment troubleshooting, and being utility efficient? How do they and facility planners calculate the costs for today’s operations and construction with an eye towards rising costs in the future?


2:45 Bruce W Kennedy Introduction of Topic and Speakers
2:50 Jeff Zynda Theoretical (Framing) Energy Savings through the Lenses of an Architect/Facility Planner
3:15 Roy E HoglundPros and Cons of Energy-Efficient Equipment: The Decision Process for Equipping a New Facility
3:35 Jori LeszczynskiCase Study on Utility Monitoring Before and After Equipment Replacement or Modification
4:05 Ethan Hildebrand Ecofriendly Cagewash Today, Not Tomorrow
4:30 James D CoxSustainability in Cagewash Staffing
4:45 AllQuestions and Answers